摘要
以外科手术切除的肝癌标本制备的细胞悬液和新建株的肝癌细胞为免疫原。籍多重免疫途经致敢ＢＡＬＢ／ｃ小鼠，经２０次融合，从近１０，０００个融合孔中，筛选出两株（ＨＡｂ２５，ＨＡｂ２７）肝癌单克隆抗体，在９４例肝癌、１４１例其他肝病组织，２６种正常组织，１２９例各组织系统的肝外恶性肿瘤组织上做了交叉反应。除ＨＡｂ２７与毛细胆管有弱的反应外，两株抗体与肝癌组织结合的阳性率高（８３．１５％，８５．１１％），仅与少数消化道肿瘤有交叉．１３１Ⅰ标记抗体进行放射免疫显像，最佳显像时间为７２ｈ，瘤／肝、瘤／血比值分别为５．０７～６．８４、１．２６～２．１３，可望为肝癌导向药物研究，提供新的载体。
In order to get more specific monoclonal antibodies(McAb)to hepatocellular carcinoma (HCC),we used cell suspensions from fresh surgical specimens and from HCC-9204 cell line as antigens,and had selected two HAb25, HAb27 ten thousands clones of hybridoma cells which were proved to be. rather specific to HCC.Their specificity were testified by ABC immunohistochemistry on the sections of 94 cases of HCC,141 cases of other liver diseases, 26 kinds of normal human tissues,129 cases of malignancies of other organs.HAb25 and HAb27 reacted with 83. 15 and 85.11 % of the HCC, respectively, without reacting to normal liver and other tissues except a slight reaction of HAb27 to bile canaliculi. On the 3rd day after iv injection of 131I-labelled antibodies into nude mice with xenograft of human HCC, the xenograft was clearly revealed by ECT with the radio-actiyity ratio of tumor/liver and tumor/blood as 5.07￣6.84 and 1.26￣2. 13 respectively.
关键词
肝细胞癌
肝肿瘤
单克隆抗体
hepatocellular carcinoma
monoclonal antibody
immunohistochemistry
radioimmunoimaging
