摘要
在以前整体及器官水平工作的基础上,本工作进一步在离体培养的胰岛细胞上观察生长抑素(somatostatin,SS)对链佐霉素(streptozotocin,STZ)诱导的胰岛β细胞损伤的保护作用。结果表明,在向培养的胰岛细胞中加入STZ前10min,加入不同剂量(0.025,0.05和0.1μg/ml)的SS,可不同程度地保护β细胞免受STZ的破坏。给药后6h,活细胞数由STZ破坏组的41.13±0.65万个/ml分别回升到49.0±2.0,53.0±1.33和53.38±1.74万个/ml;在给药后12h,活细胞数由破坏组的46.75±0.75万个/ml,分别升为53.0±1.25,57.04±2.39和63.13±0.62万个/ml,与破坏组间均有显著差异。电镜下超微结构的观察显示,破坏组胰岛内β细胞大量死亡,胞质内正常细胞器结构消失,代之以大量空泡和颗粒状物质;胞核结构不清。而保护组的β细胞结构完整,分泌颗粒轮廓典型,高尔基复合体及线粒体结构正常。用SS抗血清中和内源性SS后再给予STZ,β细胞破坏更为严重,但重新补充SS则可翻转抗血清的效果。加入钙离子载体A_(23187),对SS的保护作用无明显影响,表明这一作用可能与Ca^(2+)机制无关。
Exogenous administration of somatostatin exerted a beneficial influence directly on monolayer cultured islet B cells damaged by streptozotocin (3.0 mmol/L). Six to twelve hours following the pretreatment with somatostatin of 0.025, 0.05 and 0.1μg/ml the number of viable cells was significantly increased from 41.13±0.65×10~4 cells/ml (STZ control) to 49.0±2.0, 53.0±1.33, 53.38±1.74×10~4 cells / ml, respectively.The ultrastructural appearance of the B cells indicated that with many vacuoles and gradules occurred in the cytoplasma of these cells, normal organdies disappeared and the nuclei were obscue in structure. The pretreatment with somatostatin (0.1μg/ml) protected the B cells against streptozotocin, with mitochondria, Golgi's apparatus and granules in these cells intact.The destruction of B cells induced by streptozotocin was more severe after adding anti-somatostatin serum to neutralize the endogenous somatostatin in the culture, which was reversed by replenishment of somatostatin. Adding Ca^(2+) carrier A_(23187) did not change the protective effect of somatostatin, it seemed that there was no relationship between the protective effect of somatostatin and calcium mechanism.
出处
《生理学报》
CAS
CSCD
北大核心
1989年第4期381-387,共7页
Acta Physiologica Sinica
关键词
生长抑素
链佐霉素
胰岛细胞培养
somatostatin
streptozotocin
monolayer cultured islet cell
ultrastructure
A_(23187)
