摘要
用日本血吸虫成虫特异性抗原（ｓｊ－ＳＡＡｇ）和虫卵特异性抗原（ｓｊ－ＳＥＡｇ）免疫家兔制备免疫血清，继用ＳｅｐｈａｄｅｘＧ２００柱层析法提取家兔免疫血清中抗ｓｊ－ＳＡＡｇ和抗ｓｊ－ＳＥＡｇ的两种特异性多克隆抗体。将人“Ｏ”型血红细胞分别与上述两种抗体结合制备红细胞—抗体结合物（ＲＢＣ－Ａｂｓ），并研究了该结合物的应用方法。初步研究结果表明，实验所用载体以混合纤维素膜最佳，检测３１例急性血吸虫病人阳性率为９６．８％，２０例正常人假阳性率为５％。
In the present studies firstly, the specific antigens in adults (Sj-SAAg) and eggs (Sj-SEAg) were used to immunize rabbits for preparing immuno-sera, then, the polyclonal antibodies against Sj-SAAg and Sj-SEAg were respectively isolated from the immuno-sera by Sephadex G200 Chromatgraphy, finally, a conjugates of RBC-Abs were prepared by the combination of human type "O'' erythrocytes with the antibodies against Sj-SAAg or Sj-SEAg and the method using the conjugates as marker was studied. The preliminary study shows that mixed cellulose ester is best carrier for this detecting method. 31 acute schistosomiasis patients and 20 normal controls diagnosed by this method, positive rates are 96.8% and 5%, respectively.
出处
《安徽医科大学学报》
CAS
1994年第1期1-4,共4页
Acta Universitatis Medicinalis Anhui
基金
国家自然科学基金
青年基金
关键词
日本血吸虫病
红细胞
抗体
抗原
Schistosoma Japonicum
immunization
erythrocytes
antibodies
Sephadex G 200 Chromatography
RBC-Abs conjugates
