摘要
采用我室建立的将抗日本血吸虫特异性多克隆抗体标记在健康人“Ｏ”型血红细胞上，以国产混合纤维素膜为载体的斑点免疫结合试验（RBC－DIBA）检测日本血吸虫病人血清中循环抗原。初步结果表明该法诊断急、慢性血吸虫病的敏感性分别达到92．6％和83．1％；诊断日本血吸虫病的阳性预测值和阴性预测值为97.2％、75．8％。与斑点酶联免疫吸附试验（Dot－ELISA）的敏感性和特异性相似。
We established a dot immunobinding assay using human erythrocytes as marker- The human erythrocytes were labeled by anti-schistosoma japonicum polycIonal antibodies. The mixed nylon membranes acted as carrier. The test was used for detecting circulating schistosoma antigens. Preliminary study indicated its sensitivity as 92. 6% (50/54) and 83. 1 % (54/65) respectively in the diagnosis of acute and chronic schistosomiasis. Its positive predictive value and negative predictive value are 92.7 % and 75. 8% respectively. It showed good property for diagnusis of schlstosomiasis as Dot-ELISA. The described assay is rapid, simple and less expensive, and lends itself to use under field conditions.
出处
《中华疾病控制杂志》
CAS
1997年第1期40-41,共2页
Chinese Journal of Disease Control & Prevention
基金
国家自然科学基金!青年基金
39000093
关键词
血吸虫病
诊断
红细胞
标志物
schistosomiasis japonica
diagnosis
erythrocyte
marker
