摘要
本文采用ELISA法检测247名职业健康献血浆员中抗-HCV和HBV感染标志(HB-VM),并用PCR技术检测其中85人血清HBV-DNA的存在状况.发现抗-HCV阳性率为4.0%,HBVM阳性率为29.1%,HBV-DNA阳性率为11.8%≥40岁人群抗-HCV阳性率明显高于30岁以下人群(7.7%比0,P=0.042).HBVM阳性者抗-HCV阳性率明显高于HBVM阴性者(8.3%比2.3%,P=0.038),HBV感染与HCV感染之间存在一定的伴随关系.HBVM阳性者血清HBV-DNA阳性率明显高于HBVM阴性者(18.2%比 0,P<0.05).结论认为目前常规筛选献血员的方法不安全,建议加以改进.
serum samples from 247 occupational healthy blood donors were tested for HBV markers (HBVM)and anti-HCV by enzyme-linked immunosorbent assay (ELISA) .and 35 serum samples were detected for HBV-DNA using polymerase chain reaction (PCR) technique. In the blood donors, 4. 0%(10/247) were anti-HCV positive, 29.1% (72/24) were HBVM positive,11. 8%(10/85) were HBV-DNA positive. Out of 61 donors aged 29 years or younger, none turned positive for anti-HCV, while out of 52 donors aged 40 years or older, 4(7. 7%) donors were anti-HCV positive, showing a higher HCV-antibody prevalence among higher age group(p = 0. 042) ,but there were no difference in the positive rates of HBVM among various age groups. A-mong the HBVM positive donors,8. 3%(6/72) were anti-HCV positive,while among the HBVM negative donors, 2. 3%(4/175) were anti-HCV positive, showing a significant relation between HBV infection and HCV infection. In HBVM positive donors, 18. 2%(10/55) were HBV-DNA positive, while none was HBV-DNA positive in the HBVM negative donors,showing a higher positive rate of HBV-DNA among HBVM positive donors (p<0. 05). The positive rate of HBV-DNA among anti-HBc positive without anti-HBs donors (32%) was higher than that of among anti-KBs positive donors(6. 7%). Our observation suggested thet measurements for screening blood donors,including ALT levels and the presence of HBsAg Have not e-liminated the risk of posttransfusion hepatitis (such as hepatitis B and hepatitis C). The conclusion denotes that new criteria for screening blood donors should be developed.
出处
《重庆医学》
CAS
CSCD
1993年第3期196-197,共2页
Chongqing medicine
关键词
献血员
乙型肝炎
丙型肝炎
blood donor HBVM ANTI-HCV PCR HBV-DNA
