摘要
转基因在受精卵中的整合时间对于转基因动物的建立十分重要。采用ＷＡＰ基因调控序列指导的人Ｇ－ＣＳＦ基因为构件，对小鼠受精卵进行显微注射。对培养至１细胞期、２细胞期和８细胞期的胚胎进行ＰＣＲ检测。结果表明，三个时期转基因的检出率分别为１００％、７７．７７％和４４．４４％。说明随着培养时间的增加，转基因逐渐丢失。
Transgene was measured by using PCR method in early embryo development of fertilized eggs in this paper. 100%,77.7% and 44.4% retention of foreign gene were achieved in 1,2 and 88 cellstage resprectively. This result showed that transgene was missed in embryo development with time increasing.
出处
《中国实验动物学报》
CAS
CSCD
1997年第1期15-19,共5页
Acta Laboratorium Animalis Scientia Sinica
