摘要
采用碱性蛋白酶、中性蛋白酶、胃蛋白酶、胰蛋白酶和木瓜蛋白酶水解乳清蛋白制备ACE抑制肽,通过体外检测法测定其ACE抑制率。结果表明,碱性蛋白酶水解物的ACE抑制率最大。采用三因素二次通用旋转设计对碱性蛋白酶水解乳清蛋白的水解条件进行优化,研究了底物浓度、温度和酶与底物的质量比对ACE抑制率的影响,建立了回归方程,分析了各因素对ACE抑制率的影响,确定了最优的水解条件。
ACE inhibitory peptides were prepared from whey protein by five commercial proteases(alcalase, neutrase, pepsin, trypsin, papain), and their ACE inhibitory activity were determined in vitro by spectrophotometry assay. The alcalase hydrolysates was found to have the highest ACE inhibitor activity. The effects of protein concentration, hydrolysis temperature and enzyme to substrate ratio on ACE inhibitor activity were studied by orthogonal rotational regression design.The regression equation of ACE inhibitory activity with the protein concentration, hydrolysis temperature and enzyme to substrate ratio was established.
出处
《中国乳品工业》
CAS
北大核心
2005年第2期8-11,共4页
China Dairy Industry
基金
国家"十五"攻关项目(2002BA518A10)
关键词
乳清蛋白
ACE抑制肽
蛋白酶
水解物
whey protein
ACE inhibitory peptide
proteinase
hydrolysates
