摘要
对来自欧洲和美国的 18个刺槐种源子代进行了等位酶分析。可进行遗传分析的 7个酶系统 (Amy,Fe,L ap,Idh,Mdh,6 Pgd,Skd)中有 14个基因位点 ,其中 12个位点具有多态性。每个多态位点平均等位基因数 (A/L )变化在 1.5 6～ 3.6 7之间 ,平均基因型数 (G/L)变化在 1.6 1～ 7.11之间 ,平均等位基因有效数目 (Ae)变化在 1.0 2～ 2 .5 0之间 ,预期杂合度 (H e)变化在0 .0 2～ 0 .5 6之间。不同种源群体之间也存在较大的遗传差异 ,在 8个德国种源中 ,各群体的 A、Ae、和 H e等相对较小 ,但不同群体间差异较大。各位点等位基因频率在不同种源群体间变化也较大 ,表明德国各种源群体内遗传变异相对较小 ,但群体间差异较大。来自匈牙利和斯洛伐克的 8个种源群则相反 ,各群体的 A、Ae、和 H e等相对较大 ,而不同种源群体间差异则较小 ,各位点等位基因频率在种源群体间变化相对一致 ,表明这两个国家的种源群体内变异较大 ,但不同种源群体间差异较小。欧洲的刺槐种源并未形成明显的地理变异模式 ,而且欧洲的种源和来自原产地的美国种源相比 ,没有发现明显的差异。经过 Hardy-Weinberg平衡检测证明 ,88.4 1%位点符合 H- W遗传平衡 ,表明各群体基因频率和基因型频率保持较高的稳定性 ,且种源内的变异大于种源间变异 ,94 .
The seeds from 18 provenance populations of Robinia pseudocacia distributing in the middle Europe and the United States were collected and planted in a nursery in Waldsiversdorf, Germany. Using the leave from the two years old seedlings as materials, the genetic diversity and population genetic structure of the provenances were studied. Using starch gel and polyacrylamide gel electrophoresis, 7 enzyme systems (Amylase, Fluorescent esterase, Leucine aminopeptidase, Isocitrate dehydrogenase, Malate dehydrogenase, 6-Phosphogluconate dehydrogenase and Shikimate dehydrogenase) that could make the genetic analysis were tested. Twelve of the 14 loci studied showed detectable polymorphism. There were significant differences among the polymorphism loci. The number of alleles per locus (A/L) and the number of genotypes per locus(G/L) changed from 1.56 to3.67 and 1.61 to 7.11, respectively. The effective number of alleles per locus (Ae) ranged from 1.02 to 2.50 and the expected heterozygosity (He) from 0.02 to 0.56. Among the allele frequencies, some biggish differences were found in the same locus. At many loci, some genes in some provenance populations appeared with very low frequency or they did not appear.Large genetic differences existed in different provenance populations. The mean number of alleles per locus, the mean effective number of alleles per locus and the mean expected heterozygosity were relatively less in 8 Germanic provenance populations. However, among different provenance populations big differences and evident changes in these population genetics parameters occurred, indicating that in the 8 Germanic provenance populations, the genetic differentiation within the groups was relatively less, whereas among the groups was relatively greater. In contrast, in the 8 provenance populations from Hungary and Slovakia, these population genetics parameters within the groups were relatively greater, but differences among the groups were relatively less. The allele frequency per locus changed accordantly among the gr
出处
《生态学报》
CAS
CSCD
北大核心
2004年第12期2700-2706,共7页
Acta Ecologica Sinica
基金
国家农业部中德农业合作资助项目~~
关键词
刺槐
种源群体
等位酶
遗传结构
遗传多样性
Robinia pseudocacia
provenance population
allozyme
genetic diversity
population genetic structure
