摘要
目的:通过对正常胃组织、胃溃疡及胃癌所在胃壁组织中弹力纤维(EF)的观察,总结EF在其中的分布规律及异同并分析EF增多的原因. 方法:正常胃标本17例,胃溃疡标本7例,胃癌标本40例.进行常规取材、固定、包埋、石蜡切片、HE 染色和改良的Weigert染色,对染色结果进行观察、对比、分析. 结果:正常胃壁中EF呈规律性分布,主要位于黏膜肌层和肌层临近黏膜下层侧,纤维毛细状,数量少,分布均匀. 胃溃疡EF只在溃疡旁的黏膜肌层有显著增多,纤维增粗, 着色深,排列紊乱,当溃疡深达肌层时,其中EF增多不明显.胃癌EF在平滑肌细胞和成纤维细胞分布区域均有不同程度增多,纤维增粗,着色深,排列紊乱.胃癌组与渍疡组增多的EF数量上存在显著差异(P=0.0 042, P<0.05).胃癌组织中小血管内皮细胞增生,而胃溃疡组中未见血管内皮细胞增生现象. 结论:EF在正常胃组织、胃溃疡及胃癌周围组织中均呈规律性分布,且三者存在显著不同.胃溃疡组织EF增多可能与慢性炎症的刺激有关;胃癌周围组织中EF的增多除慢性炎症外还应该存在其他刺激因素,癌细胞还可能向外发出某种刺激因子,这些因子不仅作用于癌旁组织,还可以渗透到癌组织周围至胃壁浆膜层,作用于沿途的平滑肌细胞、成纤维细胞,使其生成EF 的能力增强,从而使其中的EF增多、增粗.癌组织还可能刺激周围组织,直接通过基因的调节使胃壁中能生成EF的细胞功能激活并增强,EF生成增多.胃壁中EF增多是一个慢性过程.胃癌组织中小血管内皮细胞增生是肿瘤相关性病变.
AIM: To study the distribution of elastic fiber (EF) in tissues of normal stomach, gastric ulcer and gastric carcinoma. METHODS: Tissue samples, including 40 cases of stomach carcinoma, 17 cases of normal stomach and 7 cases of stomach ulcer, were studied using hematoxylin and eosin (HE) staining and EF staining. The distributions of EF were analyzed under light microscope. RESULTS: In tissues of stomach ulcer, EF, with a deep staining, was mainly located in the muscularis mucosa adjacent to the ulcer lesion. No EF was observed in carcinoma zone, but a great amount was found in the smooth muscle and connective tissue around the lesion. EF was increased significantly in stomach carcinoma specimens, compared with that in normal tissues (P= 0.0042). Hy-perplasia of small vessel endothelia was observed in cancer tissues but not in ulcer tissues. CONCLUSION: The distribution patterns of EF are different in normal stomach, gastric ulcer and gastric carcinoma. The increase of EF in gastric ulcer may be related to the stimulation of chronic inflammation; while in carcinoma, in addition to the chronic inflammatory stimulation, the EF increase in the surrounding tissues may also result from augmented EF production triggered by the cancer cells indirectly through releasing stimulatory factors or directly via regulating gene expression in EF-producing cells. The increase of EF in the stomach wall lesion is a chronic process.
出处
《世界华人消化杂志》
CAS
北大核心
2005年第3期308-311,共4页
World Chinese Journal of Digestology
