摘要
目的 比较蚕蚀性角膜溃疡与正常角膜中Ⅲ型胶原、层粘连蛋白及纤维连接蛋白的定位与含量。方法 应用间接免疫荧光技术检测蚕蚀性角膜溃疡与正常角膜中Ⅲ型胶原、层粘连蛋白、纤维连接蛋白的分布。阳性结果应用图像分析系统进行定量分析。结果 Ⅲ型胶原在正常角膜中无表达 ,在蚕蚀性角膜溃疡中分布在基质溃疡处。层粘连蛋白在正常角膜基底膜微量表达 ,在蚕蚀性角膜溃疡基底膜和Bowman’s膜表达增多。纤维连接蛋白在正常角膜基底膜呈连续线状分布 ,在蚕蚀性角膜溃疡上皮层及基质溃疡处有表达 ,而在基底膜未见表达。结论 蚕蚀性角膜溃疡上皮基底膜在其发病机制中可能发挥作用 ,需要进一步深入研究。
Objective To compare the locations and levels of collagen type Ⅲ, laminin, and fibronectin in Mooren's ulcer and normal human corneas. Methods An indirect immunofluorescent technique and immunohistochemical staining were used to determine the distribution of collagen type Ⅲ, laminin, and fibronectin. The positive results were quantitatively analyzed with an image-processing system. Results Collagen type Ⅲ was not detected in the normal cornea. However, the staining could be seen in the corneal stroma close to Mooren's ulcer focus. Laminin was expressed faintly in the basement membrane of the normal cornea and the positive expression increased in the basement membrane and Bowman's membrane of Mooren's ulcer. In the normal cornea basement membrane, fibronectin was located continuously. In Mooren's ulcer basement membrane, however, fibronectin could not be found in all sections in which one expressed in epithelium and the others in stoma close to the ulcer focus. Conclusion Epithelial basement membrane may play a potential role in the pathogenesis of Mooren's ulcer.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2005年第3期254-257,共4页
Journal of Third Military Medical University
