摘要
目的建立测定复方布比卡因乳膏中利多卡因和布比卡因的含量方法。方法采用HPLC法同时检测两组分含量。色谱柱 :KromasilC18柱 (2 5 0mm× 4 .6mm ,5 μm) ;流动相 :甲醇 -醋酸盐缓冲液 (pH4 .6 ) =6 5∶35 ;流速 :1.0mL/min ;检测波长 :2 4 5nm ;进样量 :2 0 μL。 结果空白基质对两组分测定无干扰 ,在 5 0～ 30 0 μg/mL范围内线性关系良好 (r=0 .9999)。利多卡因和布比卡因的回收率分别为 10 0 .1% (RSD =1.2 8% )和 99.0 % (RSD =0 .84 % )。结论本方法快速简便、准确可靠 。
Objective To establish the method to assay the contents of lidocaine and bupivacaine from compound-bupivacaine ointment. Methods The contents of lidocaine and bupivacaine in compound-bupivacaine ointment were determined by HPLC simultaneously. The analytical column: Kromasil DOS (250 mm×4.6 mm, 5 μm), the mobile phase: methanol-acetate buffer solution (pH 4.6)=65∶35, flow rate: 1.0 mL/min, detection wavelength: 245 nm, injection volume: 20 μL. Results The blank ointment did not disturb the determination. And lidocaine and bupivacaine showed linear relation in the range of 50～300 μg/mL. The average recovery ratio of lidocaine and bupivacaine were 100.1%(RSD=1.28%) and 99.1%(RSD=0.84%) respectively. Conclusion This HPLC method was accurate, reliable, rapid and simple for assay of lidocaine and bupivacaine from compound-bupivacaine ointment.
出处
《广东药学院学报》
CAS
2004年第6期630-631,634,共3页
Academic Journal of Guangdong College of Pharmacy
