摘要
目的 应用流式细胞术检测非小细胞肺癌患者骨髓中的微转移。方法 骨髓经Ficoll梯度离心分离单核细胞后,用细胞角蛋白 19(Cytokeratin, CK19)单克隆抗体染色,应用流式细胞仪检测。结果 检测了 96例肺癌患者骨髓,发现 25例患者骨髓中有微转移。20例肺良性病变患者、10例正常人骨髓均无微转移。肺癌患者阳性率与患者病理分期、分化程度和细胞组织学类型密切相关 (P<0. 05)。结论 应用流式细胞术检测非小细胞肺癌患者骨髓中的微转移,有助于肺癌的综合治疗。
Objective To detect micrometastasis in the bone marrow of non small cell lung cancer(NSCLC) patients by flow cytometry. Methods The monotype fraction in bone marrow was isolated by Ficoll-Hypaque gradient centrifugation. The cells obtained were labeled with antibodies against CK19, then analyzed by FCM. Results Twenty five cases out of 96 patients with NSCLC were found to have micromatastasis in the bone marrow . No case was found in 20 lung benign twmor patients and 10 normal human. There was distinct correlation between detective rate of micromatastasis and pathological classification, cell differentiation of the cancer and P-TNM stage of the disease. (P<0.05). Conclusion Examination of micromatastasis in the bone marrow by FCM may be valuable in treating NSCLC.
出处
《现代肿瘤医学》
CAS
2005年第1期50-51,共2页
Journal of Modern Oncology
