摘要
利用纯培养试验方法 ,研究了栓皮栎林下凋落物中可培养的 10种主要丝状真菌对群落建群种栓皮栎 (Quercus variabilis)和林下主要伴生树种山胡椒 (L indera glauca)叶片的分解能力。结果表明 :在 10种真菌的作用下 ,9周时间内 ,栓皮栎叶片的平均失重率是山胡椒叶片的 2倍 ;两种叶片前期 (前 5周 )失重率均显著高于后期 (后 4周 )。分析结果显示叶片失重率与叶片初始木质素 /氮素、碳素 /氮素的比值成反比。根据每个菌株对每种叶片在前期和后期的重量失重率 (W)、木质素失重率 / W和木质素失重率 /全碳化合物失重率的值的相互关系 ,分解菌可以分为如下类型 :Trichoderma sp.1和 Cladosporium berbarum是对全碳化合物有一定利用能力的分解菌 ;Trichoderma sp.2、 Aspergillus fumigatus、Alternaria sp.、Penicillium sp.2对木质素、全碳化合物都有分解能力但偏向全碳化合物的分解 ,是分解能力相对较强的真菌 ;Chaetomium bostrychodes、Pestalotia sp.对木质素、全碳化合物都有分解能力并偏向木质素的分解 ,但分解能力较弱 ;Aspergillus niger、Penicillium sp.1只在试验分解前期内对木质素、全碳化合物都有一定的分解能力。不同真菌对叶片的分解能力不同 。
Decomposition of litter is a very important process for nutrient recycles in forest ecosystem. Of decomposers, fungi play a critical role in the decomposition process. In the organic layers of the soil many fungi have such roles. We are still unclear which fungus is most important. The aim of this work is to determine the decomposition ability of fungi on the decomposition process of litter in the forest of Zijin Mountain with pure incubation method. The fungi used in this work were ten main decomposing fungi, Trichoderma sp.1,Trichoderma sp.2,Chaetomium bostrychodes,Pestalotia sp., Aspergillus niger, Aspergillus fumigatus, Alternaria sp., Penicillium sp.1, Penicillium sp.2 and Cladosporium berbarum, isolated from the organic layers of the Quercus variables forest which dominated by Quercus variabilis and accompanied by Lindera glauca. Two litters of Quercus variabilis and Lindera glauca were chosen as substrate materials. The substrate leaves were cut into (2.0)cm diameter circle blades. Twelve of each kind of the dried sterilized blades (60℃) stained with the fungi solution were placed on the agar medium (2%,20ml) incubated under 20℃ and 80% moisture condition. The lignin and carbohydrate contents were determined by King and Heath's method. Carbon was analyzed by K_2Cr_2O_7-H_2SO_4 oxidation method. The total nitrogen was determined by Kjeldahl method. Samples were harvested from 5^(th) week and 9^(th) week. Triplicate was prepared for each treatment. The results indicated that during the whole period of nine weeks, the decomposition rate of Quercus variabilis driven by the ten fungi was, in general, two times to that of Lindera glauca. The weight loss of both leaves in earlier five weeks was significantly higher than in later four weeks. The decomposition rate was negatively related to the original Lignin/N (L/N) and C/N ratio. According to the weight loss of litters and the changes of Lignin/W (L/W) and Lignin/C (L/C) ratio of litters during the decomposition period, the fungi could be classified into fou
出处
《生态学报》
CAS
CSCD
北大核心
2005年第1期89-95,共7页
Acta Ecologica Sinica
基金
国家自然科学基金资助项目 ( 3 0 470 2 99)
国家 863资助项目 ( 2 0 0 3 AA2 0 90 3 0 )
教育部博士点基金资助项目 ( 2 0 0 3 0 2 840 44 )~~
关键词
真菌
落叶
分解能力
木质素
全碳化合物
fungus
litter
decomposition ability
lignin
carbohydrate
