摘要
目的 运用自体血清提取技术体外培养口腔粘膜基底细胞 (oralmucosabasalcells,OMBCs)。方法 抽取兔静脉血制备自体血清。切取幼兔颊部粘膜 ,先后经过DispaseⅡ和胰酶消化后 ,得到分散的OMBCs,分别接种到含10 %胎牛血清和 10 %自体血清的培养基。观察各代细胞增殖特点 ,计算原代细胞贴壁率、传代生长曲线及细胞倍增时间。结果 在原代细胞培养时 ,两组细胞的贴壁率无统计学差异 (P >0 .0 5 ) ;传代细胞培养时 ,自体血清培养的细胞增殖速率明显快于胎牛血清培养的细胞 (P <0 .0 5 )。结论 采用DispaseⅡ冷分离法和自体血清培养技术在短期内可以获得大量纯化的口腔粘膜基底细胞。
Objective To cultivate oral mucosa basal cells (OMBCs) in vitro applying autologous serum(AS) extractive technique. Methods The specimens harvested from rabbits' oral buccal mucosa were dissociated into isolated cells by dispase and trypsin. The isolated cells were respectively cultivated in medium with 10% FBS or 10% AS medium. Growth and morphological characteristics of OMBCs were observed. The ratio of adhesion was recorded in primary culture. The growth curve of subculture was drawn, and the population doubling time (PDT) was calculated. Results In primary culture, there wasn't statistical difference (P>0.05) in the adhesion ratio between cells cultivated in medium with 10% FBS and 10% AS. In subculture, the rate of cell multiplication of AS group was obviously faster than that of FBS group, and there was statistical difference (P<0.05). Conclusion Autologous serum culture technique together with Dispase Ⅱ could obtain a large number of pure OMBCs in a comparatively short period.
出处
《广东牙病防治》
2005年第1期19-21,共3页
Journal of Dental Prevention and Treatment
