摘要
利用抑制削减杂交和基因芯片技术获得一批黄孢原毛平革菌特异表达的EST序列,使用Phrap、EMBOSS、Blast、GENSCAN、MZEF软件,基于Linux操作系统,构建EST序列分析系统,完成了从EST和基因组Blast数据库的构建,载体序列的去除,EST序列的分类和组装,EST序列在基因组上的定位,外显子和内含子的识别以及基因预测.并通过使用perl语言结合bioperl模块写的脚本程序使分析过程自动化,从而可以快速地对大批EST序列进行分析,为克隆相关基因及研究黄孢原毛平革菌功能基因组学提供有用的信息.
A number of differentially-expressed cDNA fragments were obtained from Phanerochaete chrysosporium by using Suppression Subtractive Hybridization (SSH) and Microarray techniques and 433 of them were sequenced. To manage and analyze these EST data, based on Linux operating system, the Phrap, EMBOSS, Blast, GENSCAN, MZEF software were used to construct a platform. The platform includes constructing EST and genome databases, removing vector sequences, sorting and assembling sequences, locating on genome, identifying exons and introns, and predicting genes. Moreover, using bioperl modules, the scripts written with perl language enable analysis automatically. Results demonstrated that the robust platform could accelerate data analysis for large-scale EST sequences and offer useful information for cloning correlative genes and studying Phanerochaete chrysosporium's functional genomics.
出处
《四川大学学报（自然科学版）》
CAS
CSCD
北大核心
2005年第1期153-157,共5页
Journal of Sichuan University(Natural Science Edition)
基金
国家自然科学基金(30170513)
高等学校博士点专项基金(20010610025)
