摘要
评估幽门螺杆菌粪便抗原（ＨｐＳＡ）检测诊断ＨＰ感染的可靠性。方法：收集４３例接受胃镜检查患者的粪 便标本，用一种市售的ＥＬＩＳＡ试剂盒检测ＨｐＳＡ；以尿素酶试验、培养和涂片染色检测Ｈｐ作为“金标准”，培养和／ 或涂片染色阳性定为Ｈｐ感染。结果：“金标准”诊断Ｈｐ阳性２４例，阴性１９例。“金标准”阳性的２４例ＨｐＳＡ检测均阳 性，阴性的１９例ＨｐＳＡ检测１８例阴性和１例阳性。ＨｐＳＡ试验的敏感性为１００％（２４／２４），特异性为９４．７％（１８／１９），总的 诊断准确率为９７．６％（４２／４３）。结论：ＨｐＳＡ试验是一简便、非侵入性、准确诊断Ｈｐ感染的新方法。
Background/Aims: To evaluate the reliability of the H. pylon stool antigen (HpSA) test for detection of H. pylori infection. Methods: Stool specimens were collected from 43 consecutive patients who received upper gastrointestinal endoscopic examination for gastrointestinal symptoms. The evalu- ation of H. pylon status was based on three different tests: urease test, culture and direct microscopic examination of a Gram staining smear. A commercial kit by enzyme-linked immunosorbent assay was used to examine the HpSA in the stool. H. pylon status was defined as positive when the culture and/ or direct microscopic examination of the smear were positive. Results: Twenty four patients were diagnosed as H. pylon-positive and nineteen patients as negative. The HPSA test was positive in all 24 patients and negative in 18 patients. The sensitivity and specificity were 100% (24/24) and 94.7% (18/19), respectively. The overall accuracy rate was 97.6% (42/43). Conclusions: The HPSA test is a new, simple, non-invasive method for accurate diagnosis of current H. pylon infection.
出处
《胃肠病学》
1999年第4期213-214,共2页
Chinese Journal of Gastroenterology
