摘要
目的 评价幽门螺杆菌粪便抗原 (HpSA)快速免疫检测卡诊断Hp感染和判断Hp根除疗效的敏感度及特异度。方法 对 80例有上胃肠道症状患者的粪便标本作HpSA快速免疫卡检测 ,80例中 4 0例为接受胃镜检查而未接受过Hp根除治疗者 (A组 ) ,A组中又有 2 6例同时采用HpSA免疫卡作胃黏膜组织抗原检测 ;另外 4 0例为Hp根除治疗后的复查患者 (B组 )。诊断Hp感染和判断Hp根除的“金标准”采用Hp培养、快速尿素酶试验和Warthin Starry银染或者13 C 尿素呼气试验(UBT)进行检测 ,“金标准”是指上述 3项中 2项阳性或者Hp培养阳性 ,全部检查阴性认为Hp阴性 ,未作胃镜者以13 C UBT检查为标准。结果 80例患者中按“金标准”诊断Hp阳性 36例 ,阴性 4 4例 ;HpSA检测阳性 39例 ,阴性 4 1例 ,假阳性 3例 ,与“金标准”比较 ,其敏感度和特异度分别为 :10 0 %、93 2 % ;若A组与B组分别比较 ,A组分别为 10 0 %和 86 7% ;B组分别为 10 0 %和 96 6 % ;HpSA检测组织抗原的敏感度和特异度分别为 92 3%和 92 3%。结论 HpSA快速免疫检测卡检测粪便Hp抗原不仅可以用于临床Hp现症感染的诊断 。
Objective To evaluate the value of Helicobacter pylori (Hp) stool antigen ImmunoCard STAT (HpSA ImmunoCard STAT) in diagnosis of Hp infection and assessment of eradication of Hp Methods Stool specimens were collected from 80 patients with upper gastrointestinal symptoms, 41 males and 39 females, aged 18～73, 40 of which undergoing gastroscopy and having never received Hp eradicating regimen (group A) and another 40 of which undergoing follow up examination (group B) HpSA ImmunoCard STAT was used to detect the Hp status in the specimens of stool Rapid urease test, Hp culture, and Warthin Starry silver staining were used as 'gold standards ' to evaluate the Hp status of the biopsy specimens 13 C urea breath test ( 13 C UBT) was used as the 'gold standard' for the patients that did not undergo gastroscopy Part of the biopsy specimens of gastric mucosa underwent HpSA test too The result was considered positive when 2 of the 3 tests in the 'gold standard' were positive or culture of Hp was positive Results The sensitivity, specificity, and accuracy of HpSA test for the 80 patients as a whole were 100%, 93 2%, and 96 3%, not significantly different from those of the 'gold standard' The sensitivity, specificity, and accuracy of HpSA test for the 40 patients in the group A were 100%, 86 7%, and 95 0% respectively; and the ensitivity, specificity, and accuracy of HpSA test for the 40 patients in the group B were 100%, 96 6%, and 97 5% respectively The sensitivity and specificity of the 26 gastric biopsy specimens were both 92.3% Conclusions HpSA test by HpSA ImmunoCard STAT is a simple and accurate test in diagnosis of Hp infection and assessment of eradication of Hp
出处
《中华医学杂志》
CAS
CSCD
北大核心
2004年第14期1166-1170,共5页
National Medical Journal of China
