摘要
目的获得家兔二乙基亚硝胺诱发肝脏肿瘤的形态学资料,探讨Cmyc、p53基因和甲胎蛋白的表达情况。方法取5例家兔多结节肝癌的肿瘤性结节25个,瘤旁肝组织每例1块,进行一般病理组织学和超微结构观察。同时,以免疫组化方法检查Cmyc、p53基因和AFP的表达情况。结果和结论25个结节中,良性增生10个,高分化肝细胞癌11个,中分化肝细胞癌4个。家兔肝细胞癌的发生与Cmyc基因、AFP的过表达和p53基因的突变有关。参照肿瘤诱发过程中,增生性肝硬化、腺瘤样增生、高分化癌、中分化癌的变化顺序,在所检测的三种标志物中,Cmyc蛋白为最早出现的肿瘤标志物,p53基因突变相对较晚出现,AFP在恶性肿瘤结节中的表达率最高(933%)。
Objective To describe the pathology of liver tumors in rabbits induced by diethylnitrosamine and the expression of c myc, p53 genes and AFP in the tumors and pericarcinomatous tissues. Method 25 nodules in 5 rabbit livers and 5 pieces of pericarcinomatous tissues each from one liver were observed by light microscopy and electron microscopy, and the expressions of c myc gene, p53 gene and AFP were also observed in these tissues with immunohistochemical staining.Results and Conclusion There were 10 nodules of adenomatous hyperplasia, 11 of well differentiated hepatocellular carcinoma(HCC)and 4 of moderate differentiated HCC among the 25 nodules. The tumorigenesis of HCC in rabbits induced by diethylnitrosamine was related to the over expressions of c myc gene, AFP and the mutation of p53 gene. C myc over expression appeared first and p53 mutation the latest during the tumorigenesis of HCC. AFP over expression reached the top positive reaction ratio(93 3%) among the 3 detected parameters.
出处
《中国实验动物学报》
CAS
CSCD
2004年第4期231-234,i001,共5页
Acta Laboratorium Animalis Scientia Sinica
