摘要
目的 研究白细胞介素 13(IL 13)对小鼠支气管哮喘 (简称哮喘 )模型肺组织“钙激活的氯通道”成员gob 5和黏蛋白基因MUC5AC表达的影响 ,了解其在哮喘发病中的作用。方法 4 5只雄性BALB/c小鼠按随机数字表法分为对照组、哮喘组和IL 13组 ,每组 15只。用逆转录 聚合酶链反应(RT PCR)测定方法和免疫组化法分别检测gob 5mRNA、MUC5ACmRNA和MUC5AC蛋白在肺组织的表达。结果 对照组小鼠肺组织中gob 5mRNA表达为 0 0 0 0± 0 0 0 0 ,哮喘组为 1 136± 0 0 0 7,两组比较差异有统计学意义 (P <0 0 1) ;IL 13组小鼠肺组织中gob 5mRNA为 1 2 4 6± 0 0 0 8,哮喘组为 1 136± 0 0 0 7,两组比较差异有统计学意义 (P <0 0 1) ;对照组小鼠MUC5ACmRNA(0 0 70± 0 0 0 4 )和蛋白 (2只小鼠阳性 )表达与哮喘组 (分别为 0 16 1± 0 0 11和 7只小鼠阳性 )比较 ,差异均有统计学意义 (P分别 <0 0 1、0 0 5 ) ;经IL 13处理后的哮喘小鼠肺组织中MUC5ACmRNA (0 2 5 0± 0 0 14 )和蛋白 (13只小鼠阳性 )的表达与对照组 (分别为 0 0 70±0 0 0 4、2只小鼠阳性 )、哮喘组 (分别为 0 16 1± 0 0 11、7只小鼠阳性 )比较 ,差异均有统计学意义 (P均 <0 0 5 ) ;哮喘组和IL 13组小鼠肺组织中gob
Objective To study the influence of interleukin 13(IL 13) on the gob 5, a member of Ca 2+ activated Cl - channel and mucin gene MUC5AC expression in lungs of a murine asthmatic model, and to investigate their effects on the pathogensis of asthma Methods Forty five male BALB/c mice were randomly divided into a control group, an asthmatic group and an IL 13 group Expression of gob 5 mRNA, MUC5AC mRNA and MUC5AC protein in the lungs were detected by reverse transcription polymerase chain reaction (RT PCR) and immunohistochemical assay respectively Results No gob 5 mRNA expression in the lung was detected in the control group, while it was positive in the asthmatic group The gob 5 mRNA in the lung of the IL 13 group and the asthmatic group was 1 246±0 008 and 1 136±0 007 respectively( P <0 01) In the asthmatic group, both MUC5AC mRNA (0 161±0 011) and protein expression (7 mice positive) increased compared with the control group (0 070±0 004 and 2 mice positive, respectively) ( P < 0 01 and 0 05, respectively) IL 13 increased the expression of MUC5AC mRNA (0 250±0 014) and protein (13 mice positive) in the lung of the asthmatic group, and there was a significant difference between the IL 13 group and either the control group (0 070±0 004 and 2 mice positive, respectively) or the asthmatic group (0 161±0 011 and 7 mice positive, respectively,all P <0 05) There was a significant association between gob 5 mRNA expression and MUC5AC mRNA expression in both the IL 13 group and the asthmatic group( r =0 986 and 0 961, respectively, both P <0 05) Conclusion IL 13 is a critical cytokine in the pathogenesis of asthma, and gob 5 m
出处
《中华结核和呼吸杂志》
CAS
CSCD
北大核心
2004年第12期837-840,共4页
Chinese Journal of Tuberculosis and Respiratory Diseases
