摘要
以士的宁为模板分子,甲基丙烯酸为功能单体,乙二醇二甲基丙烯酸酯作为交联剂,甲苯和十二醇混合溶液为致孔剂,用原位分子印迹技术,合成了士的宁分子印迹整体柱。通过优化合成条件,结果显示:模板分子、功能单体与交联剂之间的比例以 1∶4∶16最佳,致孔剂中甲苯的最佳含量为 18% (V/V);对士的宁整体柱的色谱条件包括流动相组成、流速、柱温等进行了考察,并用于士的宁和马钱子碱的分离,其分离因子为 3 5。
Using strychnine as the template, methacrylic acid (MAA) as the functional monomer, ethylene glycol dimethacrylate ( EDMA) as the cross-linker, a strychnine imprinted monolithic column was prepared for the separation of the strychnine and brucine by in-situ, molecular imprinted technique. Then, the synthesis condition was optimized. The result showed the best ratio of template, functional monomer and cross-link was 1:4:16, and the toluene content in porogen was 18%. The effects of mobile phase composition, flow rate and column temperature on retention and separation factor were investigated. The maximum separation factor between strychnine and brucine was 3.5.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2005年第1期113-116,共4页
Chinese Journal of Analytical Chemistry
