摘要
用计算机辅助医学显微图像分析仪定量检测106例初发白血病患者骨髓细胞的核仁形成区相关嗜银蛋白(Ag-NORs),并与同标本中的抗人增殖细胞Ki-67阳性率、S+G2M细胞比例,DNA倍体进行比较,发现Ag-NORs与Ki-67阳性率。S+G2M细胞比例有相关关系,与DNA倍体无显著相关关系。Ag-NORs在白血病组高于正常对照组:急性白血病组高于慢性白血病;急性淋巴细胞白血病高于急性非淋巴细胞自血病;急性早幼粒细胞白血病最低。在慢性白血病中,慢性粒细胞白血病显著高于慢性淋巴细胞白血病;早幼粒细胞白血病缓解期显著低于治疗前,复发时又显著增高。结果显示:Ag-NORs可以和Ki-67免疫标记、细胞流式仪一样,作为白血病诊断,病情监测及预后判断的一项参考指标。
Argyrophilic protein associated nucleclar organizer region (Ag-NORs) of bone marrow cells on 106 cases of human leukemias were measured quantiativdy by Computer-assisted medical microimage analysis and the adta were compared with anti-human pfliferating cell ki-67 positive rate, DNA ploidy and S+G2M cell ratio in the same specimen. Results shoved that Ag-NORs had a direct bearing on ki-67 positive rate and S+G2M cell ratio, but no relationship between Ag-NORs and DNA ploidy. Ag-NORs in leukemia cells were higher than those in normal control, those in acute leukemias higher than those in chronic leukemias. Among chronic leukemias, Ag-NORs were higher in CML than in CLL, and among acute leukernias, higher in ALL than in ANLL, those in AP: L were the lowest. Ag-NORs in acute leukemias during complete remission were remarkably lower than those before treatment and while increased upon relapse.
出处
《上海第二医科大学学报》
CSCD
1993年第4期287-291,共5页
Acta Universitatis Medicinalis Secondae Shanghai
关键词
白血病
AGNOR染色
Ag-NORs leukemia proliferating antigen
