摘要
以地涌金莲吸芽为外植体,在 MS + 6-BA 1.0mg/L + IBA 0.5mg/L 培养基上培养 30d 后产生幼芽;丛芽增殖培养基为 MS + 6-BA0.3mg/L + IBA0.1mg/L,增殖系数达 2.83 以上;生根诱导最佳培养基为 MS + NAA1.0mg/L,生根率达 100%。移栽成活率 95%以上。
The haustorial bud explants from Musella lasiocarpa were cultured on MS medium supplemented with 6-BA and IBA at different concentration to induce regeneration plantlets. The results indicated that the haustorial buds can be induced on medium of MS + 6-BA 1.0mg/L + IBA 0.5mg/L after 30 days. Proliferation medium was MS + 6-BA 0.3mg/L + IBA 0.1mg/L. The reproduction coefficient was more than 2.83. The optimal medium for rooting was MS + NAA 1.0mg/L and the rooting percentage was 100%. The transplant survival percent was more than 95%.
出处
《亚热带植物科学》
2004年第4期35-36,31,共3页
Subtropical Plant Science
关键词
地涌金莲
组织培养
快速繁殖
Musella lasiocarpa
tissue culture
rapid propagation
