摘要
利用RT-PCR法扩增了经不同宿主系(乳鼠、BHK21细胞、PK15细胞)连传不同代次的口蹄疫O/China/99毒株的3A和VP1基因,并进行了核苷酸和氨基酸序列分析。结果表明,该毒株经不同宿主系传至90代后,VP1基因未发生大的变异,主要抗原位点也较稳定;而非结构蛋白3A基因却在不同宿主和代次发生突变缺失;经BHK21细胞传代后未发生缺失;经PK15细胞传代,在70～90代之间在第254～313位出现缺失;经乳鼠传代,在60～70代之间在第265～300位发生缺失,并在以后的90代毒中仍在此位缺失。这与代表性猪源流行毒O/YUN/TAW/97和O/HKN/21/70的3A基因在第276～305位发生缺失有相同之处。
3A and VP1 genes of foot-and-mouth disease virus strain O/China/99,which was serially passaged in different hosts(suckling mice?BHK21 cell?PK15 cell),were amplified by RT-PCR,and the sequences of nucleotides and amino acids were analyzed.The results were as follows:VP1 gene and the main antigenic sites were conserved through 90 passages in different hosts;however,the 3A gene of non-structural protein had deletions in different hosts and different passages.In detail,the 3A gene showed no deletion through BHK21 passages,but the deletions emerged between 70～90 passages at nucleotides 254～313 on PK15 cell,and between 60-70 passages at 265nt～300nt in suckling mice, deletions at nucleotides 265～300 emerged again on 90th passage of mice host,which was similar with swine-sourced prevailing virus O/YUN/TAW/97 and O/HKN/21/70 with deletions on nucleotides 276～305 of 3A gene.
出处
《病毒学报》
CAS
CSCD
北大核心
2004年第4期338-346,共9页
Chinese Journal of Virology
基金
国家重大基础研究发展规划项目(973项目)G1999011905
