摘要
将组织型纤溶酶原激活剂（ｔ－ＰＡ）基因ｃＤＮＡ经多次亚克隆插入到真核表达载体ｐＳＶＬ的ＳＶ４０启动子和ｐｃＤＮＡ３的ＣＭＶ启动子下游，构建了重组表达质粒ｐＳＶＬ－ｔＰＡ和ｐｃＤＮＡ３－ｔＰＡ。分别将这２种表达质粒以脂质体载体法转染ＢＨＫ２１细胞，ＥＬＩＳＡ检测结果表明，ｐＳＶＬ－ｔＰＡ和ｐｃＤＮＡ３－ｔＰＡ在哺乳动物细胞中能够表达，７２ｈ表达量分别是５２．８μｇ／Ｌ和７０．４μｇ／Ｌ。
Two expression plasmids, pSVLtPA and pcDNA3tPA, containing the tissuetype plasminogen activator (tPA) cDNA, were constructed by inserting the gene into the downstream of the SV40 promoter and the CMV promoter, respectively. These two expression plasmids were introduced into BHK21 cells with lipofection method. Supernatants collected 48 hours and 72 hours after transfection were assayed for tPA by EILSA. It was demonstracted that tPA gene expression can be detected and the expressing levels of tPA were 52.8 g/L and 70.4 g/L at 72 h for plasmid pSVLtPA and pcDNA3tPA, respectively.
出处
《中国兽医学报》
CAS
CSCD
北大核心
1999年第3期241-244,共4页
Chinese Journal of Veterinary Science
基金
国家"863"计划资助
