摘要
目的检测p16、p15及pRb在表皮肿瘤(基底细胞癌,角化棘皮瘤,脂溢性角化病等)中的表达及相互关系,以探讨它们在表皮肿瘤中发生、发展中的作用。方法用S-P免疫组化染色法检测34例基底细胞癌(BCC),12例角化棘皮瘤(KA),11例脂溢性角化病及10例正常皮肤中p16,p15及pRb蛋白的表达。结果p16、p15及pRb在基底细胞癌中均有不同程度失表达,分别为11/34(32.4%),15/34(44.1%)及10/34(29.4%);除1例脂溢性角化病pRb蛋白阴性表达外,12例角化棘皮瘤,11例脂溢性角化病及10例正常皮肤中p16、p15及pRb蛋白均呈阳性表达;基底细胞癌中,部分肿瘤p16,p15的表达与pRb蛋白的表达呈负相关,即一方的表达伴另一方的不表达。结论p16、p15及/或pRb的表达异常可能与基底细胞癌的发生、发展有关;但与表皮良性肿瘤的发生无关。
Objective To detect the expressions and relationships of p16,p15 and pRb in several neoplasms of epidermis e.g. basal cell carcinomas(BCC), keratoacanthomas,seborrheic keratoses , and to explore their roles in the development and advance of epidermal tumors . Methods The expressions of p16,p15 and pRb in 34 BCCs,12 keratoacanthomas,11 seborrheic keratoses and 10 normal skin specimens were detected by S-P immunohistochemical staining. Results Loss expressions p16、p15 and pRb were seen in 11 (32.3%); 15 (44.1%) and 10 (29.4%) cases of 34 BCCs , respectively; whereas positive expressions of them were observed in almost all 12 keratoacanthomas,11 seborrheic keratoses and 10 normal epidermises except loss expressions of pRb in 1 seborrheic keratosis;In some BCCs,there was an inverse correlation between the expressions of p16 or p15 and pRb,namely expressions of p16 or p15 were accompanied by loss expression of pRb ,and the vice versa. Conclusions The abnormal expressions of p16、p15 and/or pRb may be related to the development and advance of BCCs, but unrelated to the development of the benign neoplasms of epidermis.
出处
《国际医药卫生导报》
2004年第24期6-8,共3页
International Medicine and Health Guidance News
