摘要
目的 通过CT灌注及测量脑水肿程度 ,观察人硫氧还蛋白 (hTRX)对局灶性兔脑缺血 /再灌注损伤的保护作用。方法 采用线栓法制成一侧兔脑缺血 /再灌注模型 (栓塞 6h ,再灌注 18h) ,将 2 5只雄性新西兰白兔随机分成假手术组 ( 5只 )、缺血 /再灌注组 ( 10只 )和缺血 /再灌注 +hTRX组 ( 10只 ) ,后者给予hTRX( 0 .75mg/kg体重 ) ,其他组给予等容积的生理盐水。分别于梗死后 6h及再灌注后 18h做CT灌注图像 ,计算脑梗死面积占同侧同层大脑半球面积的百分比 ;测量脑组织含水量。结果 脑缺血 /再灌注后 ,应用hTRX治疗 ,脑梗死面积显著减少 ,脑水肿减轻。结论 重组hTRX对脑缺血
Objective To investigate the protective function of human Thioredoxin (hTRX) on cerebral ischemia/reperfusion injury of rabbit by perfusion CT and measurement of the degree of edema. Methods Unilateral cerebral ischemia/reperfusion models of rabbit were obtained by the intraluminal filament technique (embolized for 6 hours followed by reperfusion for 18 hours). Total 25 rabbits were randomly divided into sham operation group (n=5), ischemia/reperfusion group (n=10), and recombinant hTRX protein treatment group (n=10). The recombinant hTRX protein was administered to Group I/R+hTRX (0.75 mg/kg body weight), and the same volume of 0.9% sodium chloride was given to the other two groups. Perfusion CT scans were performed respectively 6 hours after ischmia and 18 hours after reperfusion, and the cerebral ischemia areas were observed, then the percents of ischmia areas account for the same cerebral areas in the same slice were calculated. Water content of the brain tissue was measured. Results After cerebral ischemia/reperfusion, the ischemia areas were significantly reduced (average reduced 30.6%), and the degree of edema was significantly lightened by using hTRX. Conclusion The recombinant hTRX protein has good effect on the treatment of cerebral ischemia/reperfusion injury. [
出处
《中国医学影像技术》
CSCD
2004年第11期1676-1679,共4页
Chinese Journal of Medical Imaging Technology
基金
广东省卫生厅科研基金资助 (A2 0 0 3734)
关键词
人硫氧还蛋白
脑缺血/再灌注
灌注
脑水肿
Human Thioredoxin (hTRX)
Cerebral ischemia/reperfusion
Perfusion
Edema
