摘要
以籼稻ZYQ8与粳稻JX17为亲本的DH群体作为研究材料 ,考察DH群体及双亲的米粒延伸率相关性状 ,并使用该群体的分子连锁图谱进行QTL分析。共检测到 14个与稻米延伸性有关的QTL ,包括 2个粒长QTL、7个饭粒长QTL和 5个米粒延伸率QTL ,分别位于第 1、2、3、5、6、7、10、11和 12染色体。所有QTL的LOD值介于2 2 6~ 9 2 5 ,分别解释性状变异的 5 31%~ 17 2 1%。在第 3染色体上的G2 4 9~G16 4、第 6染色体上的G30~RZ5 16和第 10染色体上的G10 82~GA2 2 3区间同时检测到控制饭粒长和米粒延伸率的QTL。米粒延伸性受多基因控制 ,Wx基因与位于第 6染色体上的 qCRE 6的G30~RZ5 16区间相近 。
QTLs for milled rice length (MRL), cooked rice length (CRL) and cooked rice (elongation) (CRE) were identified by using a population of 127 DH lines derived from a cross between ZYQ8 and JX17. Totally, 14 QTLs for rice (elongation) traits were detected on chromosomes 1, 2, 3, 5, 6, 7,10,11and 12. Two putative QTLs for MRL were mapped on chromosome 2. Seven putative QTLs for CRL were mapped on chromosomes 1, 6, 7, 10, 11and 12. Five putative QTLs for CRE were mapped on chromosomes 5, 6 and 10. The regions of G249~G164 on chromosome 3, G30-RZ516 on chromosome 6 and G1082~GA223 on chromosome 10 were detected simultaneously for affecting cooked rice length and cooked rice elongation. LODs of the QTLs related to rice elongation varied from 2.26 to 9.25, and their explained variations from 5.31% to 17.21%. It is indicated that cooked rice elongation was controlled by polygene and Wx-gene located on the same region with qCRE-6 was important to cooked rice elongation.
出处
《遗传》
CAS
CSCD
北大核心
2004年第6期887-892,共6页
Hereditas(Beijing)
基金
浙江省自然科学基金 ( 3 0 2 3 76)
国家自然科学基金 ( 3 0 2 70 80 8)资助~~
关键词
水稻
加倍单倍体(DH)群体
延伸性
数量性状基因(QTL)定位
Rice (Oryza sativa L.)
doubled haploid (DH) population
rice elongation
quantitative trait loci (QTL) mapping