摘要
目的观察HBcAg核酸疫苗与鼠白细胞介素12(IL12)和白细胞介素18(IL18)表达质粒联合免疫小鼠所诱导的特异性免疫应答。方法小鼠随机分为载体质粒组、HBcAg核酸疫苗组(核酸疫苗组)、HBcAg核酸疫苗+IL12组(C+IL12组)、HBcAg核酸疫苗+IL18组(C+IL18组)和HBcAg核酸疫苗+IL12/IL18组(C+IL12/IL18组)。载体质粒、HBcAg核酸疫苗、IL12及IL18表达质粒经肌内注射法免疫各组小鼠。采用酶联免疫吸附试验检测小鼠血清HBcAg特异性抗体、IgG亚类(IgG1,IgG2a)以及小鼠脾淋巴细胞培养上清液干扰素(IFN)γ含量。采用乳酸脱氢酶(LDH)释放法检测免疫小鼠特异杀伤性T淋巴细胞(CTL)活性。结果除对照质粒组外,核酸疫苗免疫的各组小鼠均能检出血清抗HBc,C+IL12组、C+IL18组和C+IL12/IL18组的抗HBc终点滴度与C组相比均明显增高(P<0.05)。各组小鼠抗HBcIgG亚类均以IgG2a占优。核酸疫苗免疫组除C+IL12+IL18组外,小鼠脾细胞培养上清液IFNγ水平均显著高于对照质粒组(P<0.01)。C+IL18组和C+IL12/IL18组小鼠脾细胞HBcAg特异性CTL活性强于其他各组。结论IL12和(或)IL18表达质粒与HBcAg核酸疫苗联合免疫,具有增强HBcAg核酸疫苗所激发的免疫应答特别是细胞免疫应答的作用。
Objective To observe the specific immune responses in mice induced by co immunization of DNA vaccine of HBcAg and plasmids encoding interleukin 12 and interleukin 18. Methods The mice were divided into following groups: vector alone, DNA vaccine of HBcAg alone, DNA vaccine of HBcAg plus plasmid of interleukin 12, DNA vaccine of HBcAg plus plasmid of interleukin 18, and DNA vaccine of HBcAg plus plasmids of interleukin 12 and interleukin 18. The mice were immunized with above DNA constructs by intramuscular injections. The levels of anti HBc and its isotypes(IgG1,IgG2a) in sera, and the level of IFN γ in supernatant of spleno lymphocyte cultures were measured by ELISA methods. CTL acti vities of spleno lymphocyte were detected with LDH release assay. Results Mice in all groups except for vector alone were sera positive for anti HBc. Comparing with group of DNA vaccine of HBcAg alone, groups of DNA vaccine of HBcAg plus plasmid of interleukin 12, DNA vaccine of HBcAg plus plasmid of interleukin 18, and DNA vaccine of HBcAg plus plasmid of interleukin 12 and interleukin 18 showed much higher end point titers of anti HBc( P <0.05). Regarding IgG isotypes of anti HBc IgG2a was dominant in each groups. The levels of IFN γ in supernatant of spleno lymphocyte cultures in all immunization groups except for DNA vaccine of HBcAg plus plasmid of interleukin 12 and interleukin 18 were significantly hig her than group of vector alone( P <0.01). CTL activities of spleno lymphocyte in groups of DNA vaccine of HBcAg plus plasmid of interleukin 18, DNA vaccine of HBcAg plus plasmid of interleukin 12 and interleukin 18 were stronger than other groups. Conclusions Co immunizations of DNA vaccine of HBcAg and plasmids encoding interleukin 12 and/or interleukin 18 can enhance the specific immune responses induced by DNA vaccine of HBcAg especially the cellular immune response.
出处
《中华传染病杂志》
CAS
CSCD
北大核心
2004年第5期310-313,共4页
Chinese Journal of Infectious Diseases
基金
卫生部科学研究基金资助项目(961347)
