摘要
利用硫酸铵沉淀、凝胶过滤层析和阴离子交换层析等方法从猪乳中分离纯化高分子量蛋白质B (HMWP-B)。 制备兔抗HMWP-B的多克隆抗体,免疫印迹结果表明,不同带型的HMWP之间存在免疫交叉反应,说明该组蛋白具有同源性,为其多态性假说提供了证据。
High molecular weight proteins (HMWP) in sow milk showed four variants of A, B, C, and D by SDS-PAGE. HMWP-B was purified by a combination of ammonium precipitation, SephadexG-200 gel filtration chromatography and High Q column anion-exchange chromatography. The rabbit anti- HMWP-B Polyclonal Antibody was then raised. Immunoblotting of HMWP in sow milk showed immunological cross reaction among these variants and indicated HMWP in sow milk are homologous. The result of this study provides a proof for the hypothesis of polymorphism of HMWP .
出处
《中国农业科学》
CAS
CSCD
北大核心
2004年第9期1369-1373,共5页
Scientia Agricultura Sinica
基金
国家自然科学基金资助项目(39770554)
关键词
猪乳
高分子量
多态性
蛋白质
同源性鉴定
Sow milk
High molecular weight protein (HMWP)
Polymorphism
Homology
