摘要
目的:进一步阐明中分子物质组分Ⅲ(MMSⅢ,MW为1090—1800dal)在尿毒症心肌病中的作用,并探讨西洋参茎叶皂甙(PQS)对其损伤心肌的保护作用的机理。方法:(1)原代心肌细胞体外培养,分离心肌线粒体。(2)在培养的第5d加入不同浓度的MMSⅢ,观察了心肌细胞乳酸脱氢酶(LDH)漏出量,细胞内丙二醛(MDA)含量,细胞内总钙含量及不同浓度MMSⅢ与线粒体孵育半小时后测线粒体钙泵活性。(3)以不同剂量的PQS作为心肌保护剂,与中浓度MMSⅢ共培养,观察LDH漏出量、MDA含量、细胞内总钙量及线粒体钙泵活性。结果:(1)建立了相对单一、稳定的心肌细胞体系,获得结构、功能良好的分离的心肌线粒体;(2)MMSⅢ浓度依赖性地使心肌细胞内MDA、总Ca^(2+)含量、LDH漏出量升高,使线粒体Ca^(2+)泵活性降低;(3)PQS能抑制MMSⅢ的上述生物学活性。结论:尿毒症血清MMSⅢ是通过对细胞内Ca^(2+)浓度影响,脂质过氧化产物聚集,造成细胞损伤,PQS通过对上述作用的拮抗起到心肌细胞保护作用。
Objective: Further understanding the role of the third Fraction of the Middle Molecular Substances (MMSⅢ) in uremic cardiomyopathy, observing protection of Panax Quinquefolium Saponin (PQS) from injury to cultured myocardial cells by MMSU and discussing its mechanism. Methods: (1) Cultured primary myocardial cells and isolated myocardial mitochondrial. (2) Treated myocardial cells which has been cultured five days with MMSⅢ of different concentration. Then, LDH released contents of medium, malondialdehyde ( MDA) and calcium contents of myocardial, cells and Ca 2+-ATP ase activity of mitochondrial were assayed. (3) Cultured myocardial cells and isolated myocardial mitochondrial. stimulation with middle concentration MMSffl (100u/dl), were treated with different concentrations of PQS, , as a protective drug, and LDH released contents of medium, MDA, calcium contents of myocardial cells and Ca 2+ - ATP ase activity were also observed. Results: (1)Established the relatively single, stable model of primary cultured myocardial cells. Obtained isolated myocardial mitochondrial with construction and function in good condition. (2) According to concentrations, MMSⅢ significantly increased LDH release, MDA and calcium contents of mycardial cells and lowed the Ca2+ - ATP ase activity of mitochondrial. (3) PQS decreasted LDH release, delined the level of MDA and calcium content and improved Ca2+ - ATPase activity .Conclusion: It is suggested that uremia serum MMⅢ caused the damage of cultured myocardial cells, and the mechanisms might be mediated effecting calcium contents of myocardial cells, inducing accumulation of lipid peroxide. PQS has notable protective effect against MMS - induced damage of cultured primary myocardial cells through mechanisms as mentioned above, and might be prospective in clinical application.
出处
《中国中西医结合肾病杂志》
2000年第2期79-81,共3页
Chinese Journal of Integrated Traditional and Western Nephrology
