摘要
目的 探讨血管内皮生长因子 (VEGF)与烟雾暴露所致大鼠肺气肿发病的关系。方法3 6只 12周龄雌性SD大鼠随机均分为烟雾暴露组 (S组 )和正常对照组 (N组 )。S组随机均分为S1、S2 、S3 组 ,分别烟雾暴露 2、4、8周 ;N组亦随机均分为N1、N2 、N3 组 ,在常氧下再饲养 0、4、8周。分别用逆转录 聚合酶链反应 (RT PCR)和改良的链霉亲合素 生物素 过氧化物酶复合物 (SABC)法检测大鼠肺内VEGFmRNA、VEGF及其受体 2 (KDR ,激酶插入区包含受体 )蛋白表达水平 ;苏木精 伊红 (HE)染色评估S组大鼠肺部病理改变 ,用平均内衬间隔 (MLI)与平均肺泡数 (MAN)评估肺气肿程度。用SPSS10 0进行方差分析、非参数检验和相关性分析。结果 (1)S组大鼠出现肺部炎症 ,至第 8周 (即S3组 )出现早期肺气肿样改变 ,S3 组的MAN较同时段N3 组显著下降 ,MLI显著增加 (P <0 0 5) ;(2 )S组大鼠肺组织VEGF表达及肺血管内皮细胞KDR表达显著下降 :S1组与N1组比较 ,VEGF189mRNA及肺泡、支气管上皮VEGF蛋白表达下降 (P <0 0 1) ;S2 组与N2 组比较 ,VEGF189、VEGF165、VEGF12 1mRNA及肺泡、支气管上皮VEGF蛋白表达均显著降低 (P <0 0 1) ;S3 组VEGFmRNA表达与N3 组比较差异无显著性 (P >0 0 5) ,但肺泡。
Objective]To explore the role of vascular endothelial growth factor(VEGF) in emphysema of rat caused by smog exposure. MethodsThirty-six 12-week male rats were randomly divided into 2 groups: a smog exposure group(S group),and a normal control group(N group ).S group rats were randomly subdivided into 3 groups:S 1 group,S 2 group,S 3 group,which were exposed to smog for 2 weeks,4 weeks,8 weeks,respectively;N group rats were randomly subdivided into 3 groups:N 1 group,N 2 group,N 3 group,which were raised in normal oxygen condition for 0 week,4 weeks,8 weeks,respectively. The expressions of VEGF mRNA and VEGF protein and kinase-insert domain containi ng receptor(KDR) protein were determined by reverse transcription-polymerase chain reaction(RT-PCR)and modified SABC immunohistochemistry assay separately. The pathological change in smog exposure rat lung was determined by HE staining. MLI and MAN were determined as an index of emphysema. Variance analysis,nonparametric analysis and correlate analysis were conducted in SPSS 10.0. Results(1)There were airway inflammation in S group rat lungs,and an early-emphysema-like change in S 3 group rat lungs:MAN in S 3 group was significantly decreased compared with N 3 group,MLI in S 3 group was significantly increased compared with N 3 group( P<0.05). (2)VEGF189 mRNA in lung and VEGF protein in alveolar and bronchial epithelium of rats in S 1 group were significantly lower than those in N 1 group( P<0.01);VEGF189 mRNA ,VEGF165 mRNA and VEGF121 mRNA of lung tissue and VEGF protein in alveolar and bronchial epithelium of rats in S 2 group were significantly lower than those in N 2 group( BX]P<0.01),too;VEGF protein in alveolar and bronchial epithelium and KDR expression in pulmonary vascular endothelium of rats in S 3 group was lower than that in N 3 group( P<0.01, P<0.01, P<0.05),but VEGF mRNA have no difference( P>To explore the role of vascular endothelial growth factor(VEGF) in emphysema of rat caused by smog exposure. MethodsThirty-six 12-week ma
出处
《中华结核和呼吸杂志》
CAS
CSCD
北大核心
2003年第11期671-674,共4页
Chinese Journal of Tuberculosis and Respiratory Diseases
