摘要
目的探讨G蛋白β3亚单位(GNB3)基因C825T多态性在早发冠心病患者中的分布情况及特点,分析其与疾病的关系。方法采用聚合酶链反应和限制性片段内切酶的方法检测了342例经冠状动脉造影证实的早发冠心病患者(冠心病组,男性275例,年龄<55岁;女性67例,年龄<65岁)及133例经冠状动脉造影排除冠心病者(对照组)的GNB3基因C825T多态性。结果GNB3基因C825T多态性在两组人群中的分布有显著性差异,冠心病组T等位基因和TT基因型频率显著高于对照组(P<005～001),C等位基因和CT、CC基因型频率两组比较均无显著性差异(P>005)。逻辑回归分析结果显示在调整了其他危险因素后,825T等位基因携带者和具有825TT基因型者早发冠心病的相对危险度增加(825T等位基因携带相对危险度=18,95%可信区间为1117～3040,P=0017;825TT基因型相对危险度=24,95%可信区间为1312～4254,P=0004),C等位基因和CC、CT基因型频率与早发冠心病的关系没有统计学意义(P>005)。结论GNB3基因C825T多态性的825T等位基因和TT基因型可能是冠心病早期发病的遗传因素之一。
Objective: This study was designed to assess whether G protein β3 subunit (GNB3) C825T polymorphisms is associated with angiographically documented premature coronary heart disease (CHD) Since high blood pressure and obesity are important risk factors for the development of CHD, the genetic variation causing hypertension may also be an important candidate gene for CHD We examined the association between GNB3 C825T polymorphisms and premature CHD Methods: Genotypes were determined with polymerase chain reaction and allele specific restriction enzyme analysis Patients with angiographically confirmed CHD ( n =342) and sex , age matched controls with no evidence of CHD ( n =133) were studied Results: The frequency of GNB3 825TT genotype (26 6 vs 15 0) and 825T allele (50 7 vs 41 1) was significantly higher in the CHD group than in the control group ( p <0 05～0 01) GNB3 825T allele carriers and those with 825TT genotype had a higher risk of premature CHD (OR for TT, 2 4; 95% CI 1 312 4 254; p =0 004; OR for T allele carries, 1 8; 95% CI 1 117 3 040; p =0 017) Conclusion: GNB3 C825T polymorphism is significantly associated with premature CHD GNB3 825TT genotype and 825T allele may be risk factors for premature CHD
出处
《中国循环杂志》
CSCD
北大核心
2004年第5期345-348,共4页
Chinese Circulation Journal
