摘要
目的分析宫颈上皮内瘤变(CIN)组织3号、10号染色体的HPV感染状态、杂合性丢失(LOH)、微卫星不稳定性(MI),以寻求CIN进展为宫颈癌的分子机制。方法选择D3S1234,D3S1300,D10S541和D10S16874个微卫星位点,应用PCR-聚丙烯酰胺-银染技术对87例CIN组织进行HPV感染状态,LOH,MI分析。结果87例CIN中,30例HPV感染阳性(34.5%);48例至少有1个位点LOH阳性(55.2%),LOH阳性率在CINⅢ明显高于CINⅠ及CINⅡ(P<0.05),在HPV感染阳性组明显高于阴性组(P<0.05);19例至少有1个位点MI阳性(37.1%),MI阳性率在CINⅢ明显高于CINⅠ及CINⅡ(P<0.05),所有MI阳性者均为LOH阳性。结论3号、10号染色体可能存在宫颈癌相关抑癌基因,LOH及MI是CIN进展为宫颈浸润癌的重要事件。
Objective:To analyze infection of human papillomoviruses(HPV),loss of heterozygosity(LOH)and microsatellite instability(MI)on chromosome3and10in cervical intraepithelial neoplasma(CIN),to find the molecular mechanism of CIN to cervical invasive carcinoma.Methods:HPV infection,LOH and MI analysis of87CIN were examined with4polymorphic microsatellite markers on chromosome3and10by polymerase chain reac-tion-polyacrylamide gel-silver stain method.Results:There were30patients HPV positive out of87CIN(34.5%)LOH occurred in48(55.2%)at least one of four loci.The incidence of LOH in CINⅢis higher than that in CINⅠandⅡ,in HPV(+)is higher than in HPV(-)(P<0.05).MI occurred in19(37.1%)at least one of four loci.The in-cidence of LOH is higher in CINⅢthan in CINⅠand CINⅡ.All of the MI positive cases are LOH positive.Con-clusions:The putative suppressor genes are likely to be located on chromosome region3and10,LOH and MI may play an important role in the development from CIN to cervical invasive carcinoma.
出处
《中国现代医学杂志》
CAS
CSCD
2004年第22期77-79,共3页
China Journal of Modern Medicine
关键词
宫颈上皮内瘤变
人乳头瘤病毒
杂合性丢失
微卫星不稳定性
遗传不稳定性
cervical invasive carcinoma
human papillomovirues
loss of heterozygosity
microsatellite instability
genetic instabilities
