摘要
传统的放射或超声检查早期诊断胰腺癌非常困难。目的:研究胰腺良、恶性病变中的K-ras基因12密码子点突变情况,探讨K-ras基因点突变对早期胰腺癌的诊断价值。方法:取23例胰腺癌和12例胰腺良性病变标本,经DNA提取后,行半巢式聚合酶链反应(PCR),扩增产物借助聚丙烯酰胺凝胶电泳检测有无K-ras基因点突变。结果:胰腺癌和胰腺良性病变石蜡包埋组织的K-ras基因12密码子点突变率分别为65.2％(15/23)和33.3％(4/12)(P<0.05)。结论:K-ras基因12密码子点突变的检测可能有助于胰腺癌,尤其是早期胰腺癌的诊断。
Early diagnosis of pancreatic adenocarcinoma by the traditional radiographic or ultrasonographic methods is very difficult. Aims: To explore the K-ras gene point mutation at codon 12 in both malignant and benign pancreatic diseases, and to evaluate its diagnostic value in early pancreatic adenocarcinoma. Methods: Seminested polymerase chain reaction (PCR) was performed in the pancreatic specimens of 23 patients with pancreatic adenocarcinomas and 12 benign pancreatic diseases after DNA extraction. K-ras gene point mutation of the amplified product was detected by polyacrylamide gel electrophoresis. Results: The rates of K-ras gene point mutation at codon 12 was 65.2% (15/23) and 33.3% (4/12), respectively (P<0.05) in paraffin embedded pancreatic adenocarcinoma and pancreatic tissues of benign pancreatic diseases. Conclusions: Detection of K-ras gene point mutation at codon 12 may be helpful for the diagnosis of pancreatic adenocarcinoma, especially at its early stage.
出处
《胃肠病学》
2003年第4期207-209,共3页
Chinese Journal of Gastroenterology
