摘要
目的 建立子宫内膜异位症 (内异症 )裸鼠模型 ,并对其相关生物学行为进行研究。方法 取人分泌晚期子宫内膜 ,剪碎 ,置入裸鼠盆、腹腔不同部位 ,内异症 (EM组 )和非内异症 (NEM组 )裸鼠各 2 4只 ;对照组 :3只裸鼠 ,同法将人大网膜组织置入裸鼠盆、腹腔不同部位。各组裸鼠分别于置入后第 5、15、30天随机脱颈椎处死 ,观察异位病灶生长情况 ,并留取异位病灶 ,在光镜及透射电镜下观察其形态学变化。RT PCR法检测在位内膜及异位病灶血管内皮生长因子 (VEGF)、基质金属蛋白酶 9(MMP 9)mRNA表达 ,免疫组化链霉素抗生物素蛋白 过氧化物酶连接法检测异位病灶雌、孕激素受体表达。结果 内膜置入 5d ,即见异位病灶牢固黏附 ,血液供应丰富。光镜下见腺体细胞及散在间质细胞 ,且随时间延长 ,异位病灶与裸鼠组织融合越明显。 15d时 ,电镜下可见异位病灶内炎性细胞增生、浸润最明显 ,30d时腺细胞中细胞器消失 ,但仍可见分泌颗粒 ,核染色质聚集 ,并有凋亡趋势。与在位内膜比较 ,异位病灶VEGF、MMP 9mRNA表达增强 (P <0 0 5 ) ,异位内膜置入第 15天时表达最强 (VEGF :EM组为 1 11± 0 13、NEM组为 1 10± 0 11;MMP 9:EM组为 1 0 0± 0 11、NEM组为 0 99± 0 0 8) ,第 30天时有所下降 (VEGF :EM组为 0 85± 0
Objective To establish an experimental endometriosis model using nude mouse as a xenograph ic host for relative biological behavior study of endometriosis. Methods Nude mice of experimental group were implanted with the late secretory endometri um of patients with endometriosis (n=24) or without endometriosis (n=24) into pelvic and abdominal cavities. Nude mice of control group (n=3) were implanted with the greater omentum. Nude mice were killed at 5, 15 and 30 d ra ndomly to observe the growth of endometriotic lesions. The morphological change s of endometriotic lesions from different sources and at different time points o f growth were examined by light microscopy and electron microscopy; the expressi ons of vascular endothelial growth factor (VEGF) and matrix metalloproteinase-9 (MMP-9) of eutopic endometrium and endometriotic lesions were detected by RT- PCR method, and the expressions of steroid hormone receptors by immunohistochemi stry method. Results Endometriotic lesions were found from 5 d with rich blood supply, endometrial g lands and stroma under light microscope. And the adhesion to mouse tissues beca me more severe with time. Under electron microscope, proliferation and infi ltration of inflammatory cells were most striking at 15 d. At 30 d the organell ae of gland cells were disappeared and there was nuclear chromatin aggregation w ith apoptosis tendency, but secretory granules still could be seen. The express ions of VEGF and MMP-9 mRNA were increased in endometriotic lesions compared wi th eutopic endometrium (P<0.05), and were strongest at 15 d(EM group:1. 11±0.13/1.00±0.11;NEM group:1.10±0.11/0.99±0.08)and decreased at 30 d(EM group:0.85±0.11/ 0.77±0.13;NEM group:0.86±0.14/ 0.76±0.1 1). Immunochemistry revealed the positive rate of estrogen and progestogen rec eptors at 30 d (EM group:4/8 and 4/8;NEM group:5/8 and 4/8)was lower than t hat at 5 d (EM group:7/8 and 7/8;NEM group:8/8 and 7/8) and 15 d (EM group : 7/8 and 7/8;NEM group:7/8 and 8/8),and the expressions of ste
出处
《中华妇产科杂志》
CAS
CSCD
北大核心
2004年第11期759-762,i004,共5页
Chinese Journal of Obstetrics and Gynecology
基金
辽宁省教育厅 2 0 0 2年度高等学校科学研究基金资助项目 (2 0 2 0 13 117)
关键词
异位
病灶
表达
置入
裸鼠
孕激素受体
体表
细胞器
腺细胞
分泌
Endometriosis
Disease model, animal
Mic e,nude
Vascular endothelial growth factor A
Gelatinase B
Gene expression
