摘要
目的 :探讨植物血凝素 (PHA)对细胞因子诱导的杀伤细胞 (CIK)增殖和细胞毒作用的影响。方法 :分离健康人外周血单个核细胞 ,用自体血浆培养CIK细胞和PHA先刺激 2 4h的PHA CIK细胞。用甲基四唑盐(MTT)法检测两种细胞对K5 6 2细胞、食管癌细胞和初治急性白血病患者骨髓幼稚细胞的细胞毒作用。结果 :两种细胞体外增殖能力较强 ,且PHA CIK细胞强于CIK细胞 (P <0 .0 5 )。两种细胞对不同靶细胞均有较强的杀伤能力。在效靶比为 2 0∶1、10∶1时 ,PHA CIK细胞比CIK细胞对K5 6 2细胞和初治急性白血病患者骨髓幼稚细胞的杀伤作用强 (P <0 .0 5 )。结论 :PHA增加CIK细胞的增殖能力和抗肿瘤活性 ,可作为生物治疗应用于临床。
Objective:To study the effect of phytohemagglutinin (PHA) on cytokine induced killers' (CIK) proliferation and cytotoxicity.Methods:Peripheral blood mononuclear cells (PBMNCs) from healthy donors were divided into two groups. 10% autologus plasma was used to incubate cells. In one group CIK cells were cultured by traditional method, and in the other group phytohemagglutinin (PHA) was added to stimulate PBMNCs for 24 hours, then cultured like incubating CIK cells. MTT method was used to evaluate their cytotoxicity to different target cells.Results:The proliferation rate of CIK and PHA-CIK cells were high. There was significant difference between them (P< 0.05). Each group cells had high cytotoxicity. At the same higher effect/target ratio PHA-CIK cells' cytotoxicity was stronger than CIK cells' when targets were K562 cells (P< 0.05).Conclusions:PHA-CIK cells had stronger proliferation and cytotoxicity than CIK cells. The result provides an experimental basis for biotherapy.
出处
《临床血液学杂志》
CAS
2004年第6期340-342,共3页
Journal of Clinical Hematology
