摘要
目的 研究FK5 0 6结合冷藏保存应用于同种异体神经移植后对神经再生的实验效果。方法 60只雌性Wistar大鼠做为受体动物模型 ,按移植物的不同分为 4组 ,每组 15只大鼠。A组 :新鲜同种异体神经移植组 ;B组 :冷藏保存同种异体神经移植组 ;C组 :冷藏保存同种异体神经移植组 ,同时服用FK5 0 6,剂量为 ( 2mg/kg) 1·d-1;D组 :冷藏保存同种异体神经移植组 ,同时服用FK5 0 6,剂量为 ( 0 .5mg/kg) -1·d-1。于术后 4、8、10周各组分别行大体观察 ,测定比目鱼肌肌湿重 ,运动神经传导速度 (MNCV)和运动动作电位 (CMAP)的波幅及组织学变化。结果 术后 8、10周时 ,4组中以C组神经再生最好 ,D组神经再生好于A、B组 ,但与C组相比则较差。轴突计数、肌湿重的统计学分析 ,有明显差别。结论 冷藏保存预处理同种异体神经移植后可以加速神经再生及功能恢复。
Objective To study the effects of FK506 and cold preservation on nerve regeneration after transfer of allografts in rats. Methods Sixty female Winster rats were randomized into 4 groups: fresh allografts (group A), allografts treated with cold preservation (group B), allografts with cold preservation combined with FK506 (2mg/kg) -1 ·d -1 (group C), allografts with cold preservation combined with FK506 ( 0.5mg/kg) -1 ·d -1 (group D). On 4,8, and 10 weeks after operation, the specimens were observed under naked eyes. The wet muscle weight of soleus muscle, the motor nerve conduction velocity(MNCV), the amplitude in compound muscle action potential and histology examination were done respectively.Results On postoperative 8 and 10 weeks, the result of nerve regeneration was best in group C among the 4 groups. The result in group D was better than that in group A and B. There were statistically significant difference in the number of axons and wet muscle weight. Conclusion The cold preserved nerve allografts can efficiently promote function recovery of nerve regeneration.
出处
《中华手外科杂志》
CSCD
2004年第3期137-139,共3页
Chinese Journal of Hand Surgery
基金
河北省医学科学研究重点课题计划资助项目 (0 30 66)
