摘要
目的 研究预投D -青霉胺 (DPA)和二巯基丙磺酸钠 (DMPS)对急性汞氧化损伤的保护作用 ,进一步探讨无机汞氧化损伤的作用机制。方法 4 8只Wistar大鼠随机分成 6组。第 1组以 5ml/kg皮下注射 0 9%氯化钠溶液 ,第 2～ 4组分别皮下注射 0 75 ,1 5 ,2 5mg/kgHgCl2 溶液。第 5 ,6组大鼠分别腹腔注射DMPS、DPA 2 0 0 μmol/kg ,2h后再投与 2 5mg/kgHgCl2 溶液。染毒 12h后 ,收集 12h尿样 ,测定尿汞含量。染毒 4 8h后 ,切取肾脏和肝脏 ,测定丙二醛 (MDA)和谷胱甘肽 (GSH)含量及谷胱甘肽过氧化物酶(GSH Px)活力。结果 DMPS可显著降低肾脏MDA含量 ,而DPA对肾脏MDA含量没有影响。DMPS和DPA对肝脏MDA的变化没有影响。DMPS和DPA两干预组在肾脏和肝脏中GSH含量和GSH Px活力都明显高于 2 5mg/kg染汞组 ,差异有显著性。DPA能显著降低肾脏汞的含量。结论 DMPS可显著减轻汞在肾脏的氧化损伤 ,但对肝脏没有影响。DPA对汞在肾脏和肝脏氧化反应都没有影响。DMPS能减少肾脏和肝脏GSH的耗竭 ,而给予DPA只能防止肾脏GSH的耗竭 ,对肝脏GSH影响不大。DMPS和DPA能防止GSH Px耗竭。DPA可减少汞在肾脏的蓄积量 ,致使汞分布到其他组织器官中 ,而DMPS不能引起汞的这种分布。
Objective To study the attenuating effect of 2,3 -dimercapto-propane-1-sulfonate(DMPS)and D-penicillamine (DPA) on acute toxicity of mercury and its mechanism and to further probe into the mechanism of oxidative damage induced by inorganic mercury.Methods 48 Wistar rats were randomly divided into 6 groups.The first group was injected subcutaneously with 0.9% saline.The rats in next three groups were injected subcutaneously with 0.75,1.5,and 2.5 mg/kg mercuric chloride (HgCl 2) respectively.The rats in the 5th and 6th groups were pretreated either with DMPS or with DPA followed by injections of 2.5 mg/kg HgCl 2 to both groups 2 hours later.12 hours later,the urine samples were collected and the urinary Hg determined.All rats were sacrificed 48 hours later.The contents of malondialdehyde (MDA) and glutathione(GSH), and activities of glutathione peroxidase (GSH-Px) were determined.Results Although DMPS diminished significantly the content of MDA in the kidney,DPA showed no effect on MDA.The change of hepatic MDA content didn't attribute either to DMPS or to DPA.Both the contents of GSH and activities of GSH-Px of the DMPS and DPA pretreated groups were significantly higher in the kidney and liver than those of 2.5 mg/kg mercury group.DPA reduced the content of renal mercury significantly.Conclusions DMPS significantly mitigates the degree of lipid peroxidation in the kidney,but not in the liver.DMPS could reduce the depletion of GSH in both the kidney and the liver;and DPA only diminishes the depletion of the renal GSH,but not having any impact on hepatic GSH.Both DMPS and DPA could prevent the depletion of GSH-Px;and DPA reduces the accumulation of mercury in the kindney significantly,resulted in distribution of mercury to other tissue or organ,however,the change doesn't result from DMPS.
出处
《工业卫生与职业病》
CAS
CSCD
北大核心
2004年第6期342-345,共4页
Industrial Health and Occupational Diseases
