摘要
目的 分析转化生长因子 β1(TGF β1)在原发性肝癌 (PLC)诊断和判断预后方面的价值。 方法 以酶联免疫吸附试验法 (ELISA)测定肝癌、肝硬化 ,慢性肝炎、急性肝炎、继发性肝癌 (SHC)、消化道其它肿瘤和正常对照者的血浆TGF β1水平 ,并分析在PLC诊断方面的临床意义。结果 PLC组血浆TGF β1水平 ( 2 2 1± 1 19μg/L)均显著高于 (P <0 0 5 )正常对照组 ( 0 67± 0 11μg/L)、肝硬化 ( 0 95± 0 2 3 μg/L)、慢性肝炎 ( 0 98± 0 2 0 μg/L)、急性肝炎 ( 0 72± 0 3 7μg/L)、SHC( 0 89± 0 19μg/L)和消化道其它肿瘤 ( 0 82±0 0 9μg/L)。如以血浆TGF β1水平 1 2 μg/L为界 ,其诊断PLC的灵敏性和特异性分别为 89 5 %和 94 0 %。并且与血清AFP浓度、肿块大小均无相关性 ,与AFP联合检测可提高PLC诊断阳性率。结论 资料提示TGF β1过度表达与肝癌形成有关 ,血浆TGF
Objective To investigate the clinical values of the level of plasma TGF-β1 in diagnosis and prognosis estimation of primary liver cancer (PLC).Methods The plasma levels of TGF-β1 expression were analyzed with enzyme-linked immunosorbent assay (ELISA) in patients with PLC, liver cirrhosis (LC), chronic hepatitis (CH), actute hepatitis (AH),secondary hepatocellular carcinoma (SHC), other gastroenteric cancers, and health controls. The clinical significance was investigated in PLC diagnosis.Results The plasma TGF-βl levels in PLC patients were 2.21+1.19μg/L (mean±SD) and significantly higher (P<0.05) than those in normal subjects (0.67±0.11μg/L), LC (0.95±0.23μg/L), CH (0.98±0.20μg/L), AH (0.72±0.37μg/L), SHC (0.89±0.19μg/L), and other gastroenteri ccancers (0.82±0.09μg/L),respectively. The sensitivity and specificity of plasma TGF-β1 levels were 89.5% and 94.0% in diagnosis of PLC with 1.2μg/L as normal up-limitation, respectively. No significant correlation was found between the plasma TGF-β1 levels in patients with PLC and serum AFP levels or the tumor sizes. Combining analysis of plasma TGF-β1 levels and serum AFP could increase the detection rate for PLC.Conclusions The presents data suggests that the overexpression of TGF-β1 is probably correlated with the development of PLC, and analysis of plasma TGF-β1 is helpful to diagnose PLC and surveillance of its matastasis.
出处
《胃肠病学和肝病学杂志》
CAS
2004年第5期502-504,共3页
Chinese Journal of Gastroenterology and Hepatology
基金
江苏省医学重点人才项目 (RC2 0 0 31 0 0 )部分资助
