摘要
目的分析儿童急性淋巴细胞白血病(ALL)的免疫表型特点。方法用一组相关单克隆抗体和流式细胞技术间接免疫荧光法对85例儿童ALL进行免疫分型。结果 T系ALL占28.2％,B系ALL占61.2％,T/B急性混合细胞白血病(AMLL)8.2％,其中髓系抗原表达率21.2％。各组间L_1、L_2比率、染色体异常及治疗完全缓解(CR)率均无显著性差异(P>0.05);T-ALL中CD_3平均阳性率较CD_2、CD_7低(P<0.01,P<0.05),B-ALL中CD_(22)阳性率明显低于CD_(10)、CD_(19)(P<0.01)。结论 ALL免疫分型与FAB分型、染色体核型变化无相关性;系列特异性单抗CD_3、CD_(22)在诊断T-ALL、B-ALL中起决定作用;ALL髓系抗原阳性与治疗CR率无关。
Objective To analyse the immunophenotype of childhood acute lynphoblastic leukemia(ALL). Methods 85 cases of childhood acute lymphoblastie leukemia(ALL)were immunologically classified by a se- ries of lineage-associated monoclonal antibodies and folwcytometric indirect immunofiuorescence.Results The percentage of T-ALL was 28.2%,while that of B-ALLand T/B acute mixed-lineage leukemia(AMLL)was 61.2% and 8.2% respectively.The rate of myeloid antigen exprission was 21.2%.There was no significant dif- ference in rate of Lland L_2,chromosome abnormality and complete remission(CR)Between groups(P>0.05)The positive rate of CD_3 in T-ALL was lower than that of CD_2 and CD_7(P<0.01,P<0.05);the positive rate of CD_(22)in B-ALL was lower than that of CD_(10) and CD_(19)(P<0.01).Conclusion Immunphenotype was not correlated with FAB subtype or chromosome abnormality in children ALL;the lineage-specific monoclonal antibodies CD_3 and CD_(22)were important to diagnose T-ALL and B-ALL.Myeloid antigen expression was not associated with CR rate.
出处
《基层医学论坛》
2004年第10期875-876,共2页
The Medical Forum
