摘要
目的 探讨在肝癌SMMC - 772 1细胞中蛋白激酶B(PKB)对 β 连环蛋白 (β catenin)的调控作用。 方法用WesternBlot和RT PCR等研究在肝癌SMMC - 772 1细胞中激活或抑制PKB后 ,β catenin基因及蛋白水平的变化。结果 以H2 O2 活化PKB ,则SMMC - 772 1细胞胞质内游离的 β catenin水平明显升高 ,而与E cadherin结合的 β catenin水平没有变化 ,β catenin的mRNA表达也未见明显改变 ;用Wortmannin抑制PKB的活性 ,可使胞质内 β catenin的含量下降。 结论 肝癌SMMC - 772 1细胞中PKB可以调控胞质中游离的 β catenin ,这为深入研究 β
Purpose: To study the regulation of β-catenin by activating and inactivating protein kianase B (PKB) in human hepatocellular carcinoma cell line SMMC-7721. Methods: The effects of H2O2 and Wortmannin on levels of PKB protein, phosphorylation of PKB and β-catenin protein were studied by Western blot. The levels of β-catenin mRNA treated with H 2O2 and Wortmannin were detected by RT-PCR. Results: In our research, when SMMC - 7721 cells were treated with H2O 2, which can activate PKB/Akt via PI3K pathway, the protein level of free β-catenin in cytoplasma was up-regulated, while there's no change in mRNA expression of β-catenin. Contrarily, when the cells were treated by PI3K special inhibitor, Wortmannin, β-catenin in cytoplasma was down-regulated. Conclusions: These results suggest that PKB can regulate free β-catenin in human hepatocellular carcinoma cell line SMMC-7721.
出处
《复旦学报（医学版）》
EI
CAS
CSCD
北大核心
2004年第5期445-448,共4页
Fudan University Journal of Medical Sciences
基金
国家自然科学基金项目 ( 3 9970 3 3 8)
上海市教委重点 ( 8990 80 6)资助项目
