摘要
目的 :比较Leptin在胃溃疡及胃癌中表达的差异性。方法 :分别通过胃镜和手术获得胃溃疡及胃癌的样品标本 ,用West ern blot法及免疫组化方法 (DAB显色法 )分别检测人胃溃疡组织及胃癌组织中Leptin蛋白的表达情况。结果 :胃癌组织、癌旁组织、溃疡周边组织中均可测量到Leptin蛋白。免疫组化染色结果显示Leptin在癌组织中和胃溃疡周边黏膜均有表达 ,在胃癌组织中表达较强。结论 :Leptin在胃癌组织中表达高于胃溃疡周边组织 ,且两者表达有明显差异性 。
Objective:To study the difference of leptin expression in the gastric ulcer and carcinoma cells.Methods:Samples of the ulcer surrounding mucosa were obtained under gastroscopy.Samples of carcinoma and the surrounding mucosa are obtained from operation.Western-blot was performed to measure leptin protein level in gastric mucosa cells.Immunohistochemistry (DAB) was used to locate of leptin potein.Results:Leptin protein was mensurated in ulcer surrounding mucosa cells and the gastric carcinoma cells.Immunohistochemistry locate leptin in gastric mucosa cell and carcinoma cell.Conclusion:Leptin proteins expression in human gastric cancer are little more than in gastric ulcer.There is strong difference between gastric carcinoma and gastric ulcer.
出处
《临床消化病杂志》
2004年第5期204-205,233,共3页
Chinese Journal of Clinical Gastroenterology
关键词
LEPTIN
胃溃疡
胃癌
Leptin
Gastric ulcer
Gastric carcinoma
