摘要
采用小牛胸腺DNA修饰金电极并用纳米金增强了DNA在电极上的吸附量.Co(bpy)3+3被用作电子媒介体来表征通过恒电位法吸附在电极表面的DNA的变化过程,并通过循环伏安法测定Co(bpy)3+3在电极上的富集量;同时,考察了DNA修饰金电极在不同pH值及不同温度下的稳定性,发现纳米金使DNA修饰电极稳定性增加.
We use calf-thymus DNA(CT DNA) to modify the gold electrode and colloid Au to enhance the DNA immobilization amount on the electrode. The DNA modified electrode was electrochemically characterized with Co(bpy)^(3+)_3, a electroactive DNA-binding complex, as an indicator. The amount of Co(bpy)^(3+)_3 enriched by the layers of DNA LSV(linear sweap voltammetry) adsorbed at the electrode was estimated from the peak charge of 5 mmol/L Co(bpy)^(3+)_3 reduction at the electrode obtained by cycle voltmmetry(CV). The stability of the DNA-modified electrode was investigated and the results obtained exhibit that the colloidal Au make the DNA-modified gold electrode stable to alkali, acid and hot water compared to the bare gold electrode.
出处
《西南师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2004年第5期789-794,共6页
Journal of Southwest China Normal University(Natural Science Edition)
基金
国家自然科学基金资助项目(29705001)
教育部优秀青年教师资助计划(教人司2002-40)
重庆市自然科学基金资助项目(2002-7477).
