摘要
目的 观察氯胺酮对细胞能量代谢障碍、兴奋性氨基酸中毒和氧自由基损伤三种不同性质脑损伤的作用。方法 制备大鼠皮层和海马脑片 ,培养胎鼠皮层神经元 ,建立缺氧缺糖 (OGD)、谷氨酸和过氧化氢 (H2 O2 )三类损伤模型 ,采用脑片TTC染色和神经元MTT比色法 ,酶标仪测定4 90nm处吸光度 (A值 ) ,定量考察损伤程度和氯胺酮的作用。结果 三类损伤能够明显降低皮层、海马脑片TTC染色和神经元MTT比色吸光度A值 (P <0 0 5 )。氯胺酮对正常孵育脑片和培养神经元染色无影响 ,明显减轻OGD与谷氨酸损伤脑片和神经元所致A值降低 (P <0 0 5 ) ,对H2 O2 损伤所致A值降低无作用。结论 氯胺酮保护细胞能量代谢障碍和兴奋性氨基酸中毒所致脑损伤 ,对氧自由基损伤无保护作用。
Objective To evaluate the effects of ketamine on the brain injuries induced by metabolism impairment,excitotoxitic of excitatory amino acids or oxygen-derived free radicals. Methods Models of oxygen-glucose deprivation(OGD),glutamate,hydrogen peroxide(H 2O 2) injuries on rat brain slices(cortex and hippocampus)and cultured fetal rat cortical neurons were established.Injuries and the effects of ketamine were evaluated by ELIAS reader of absorbance(A value)at 490 nm from 2,3,5-triphenyltetrazolium chloride(TTC)staining and 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide(MTT)colorimetric assay.Results Three kinds of injuries could significantly decrease A value of TTC staining and MTT assay.Ketamine at both clinical and higher concentrations could significantly attenuate the decreases of TTC staining in brain slices and MTT assay in the cultured neurons induced by ODG and glutamate injuries,but had no effects on the H 2O 2 injury.Conclusion Ketamine has a protective effect against the brain injuries induced by OGD and glutamine,but not H 2O 2.
出处
《临床麻醉学杂志》
CAS
CSCD
2003年第12期734-737,共4页
Journal of Clinical Anesthesiology
