摘要
目的 本文旨在通过建立肌亚部神经挤压损伤的模型 ,以肌内神经、肌梭 ,运动终板带、肌湿重等为参数观察神经的损伤及修复。方法 1.用sihler’s肌内神经染色法染肌内神经。 2 .用乙酰胆碱脂酶整肌染色法观察肌运动终板带变化。 3.用HE染色法观察肌梭。结果 实验组手术后两周肌亚部轻度萎缩 (肌湿重P <0 0 5 ) ,运动终板带变绍颜色变浅 ;肌内神经连续性存在 ,但三级末梢染色浅淡甚至消失 ;肌梭形态无明显变化。手术后五周到六周肌湿重增加 ,肌内神经着色深 ,三级末梢清晰可见 ,肌外观基本恢复正常。与其他亚部相比 ,腓肠肌内侧亚部各参数恢复较快。手术后八周所有各参数恢复与对照组比较无显著性差异 (肌湿重、肌梭密度p >0 0 5 )。结论 1.家兔腓肠肌外侧头各亚部在神经挤压损伤六周后肌内神经分支分布恢复至正常形态 ,神经末梢感受器及运动终器恢复。 2 .在亚部水平神经被挤压后 ,神经的再生与修复速度较整肌更早 。
Objective To discuss the nerve regeneration by observe the changes of morphology of intramuscular nerve,motor end plate band and muscle spindl after nerve crush in compartment level of lateral gastrocnemius of rabbit.Methods 1.Sihler’s intramuscular neural straining technique was used to observe the repairing degree of nerve injuried in different periods of post-operation.2.AChE straining technique with integrated muscle was used to investigate the change of MEP band in different rabbit models.3.HE straining technique and stereology method were used to study the varieties of the muscle spindle in morphology.Results 1.Two weeks later muscles withered lightly(wet weight P<0.05)in crushed group;the color of MEP band became faint and thin;third-class nerve branches,almost disappeared in muscle,the number and shape of muscle spindles were not changed.2.Six weeks later,muscle wet weight increased,third-class nerve branches could be seen again.Additionally,nerve repair of the intermediate head is the fastest than the others’.3.Eight weeks later,the changes mentioned above disappeared on the whole.Conclusions 1.Six weeks post-crushing,characteristics of intramuscular nerve became the same to control’s.2.Recovery of compartment was rapider than that of the whole muscle after the nerves were crushed respectively.
出处
《四川解剖学杂志》
2004年第3期199-202,共4页
Sichuan Journal of Anatomy
关键词
兔
腓肠肌
肌亚部
神经挤压
肌内神经
运动终板
肌梭
intramuscular nerve
motor end plate
muscle spindle
nerve crush
compartment
