摘要
目的 检测Eales’病患者房水及玻璃体中血管内皮生长因子 (VEGF)含量 ,探讨VEGF对Eales’病预后的影响。方法 应用酶联免疫吸附测定法对 16例Eales’病患者行玻璃体切割术时取得的房水、玻璃体标本进行VEGF检测 ,并对同期 6例黄斑裂孔手术标本中的VEGF做同样检测作为对照。结果 Eales’病患者房水、玻璃体中VEGF质量浓度分别为 ( 1 15 6± 0 2 0 4)、( 1 2 10± 0 2 44 )ng/ml ,较对照组 ( 0 189± 0 0 3 8)、( 0 2 0 1± 0 0 5 5 )ng/ml明显增高 ,两组间差别有非常显著性意义 (P <0 0 1)。结论 Eales’病患者房水、玻璃体中VEGF质量浓度明显增高 。
ObjectiveTo investigate possible effect of vascular endothelial growth factor(VEGF)in aqueous humor and vitreous on the development of vitreous hemorrhages in patients with Eales'disease.MethodsThirty two samples of aqueous humor and vitreous were obtained from 16 patients with Eales'disease during intraocular surgery.Levels of VEGF were measured by using enzyme linked immunosorbent assay(ELISA).Eleven samples of ocular fluid from 6 patients with macular hole were detected by the same methods as control.ResultsThe VEGF concentration in aqueous humor(1 156±0 204) ng/ml and vitreous(1 210±0 244)ng/ml with Eales'disease were significantly higher than those in the control group(0 189±0 038 ng/ml,0 201±0 055 ng/ml)( t =16 876, P <0 001; t =12 094, P <0 001,respectively).ConclusionsThe patients with Eales'disease have higher level of VEGF in ocular fluid and VEGF might play an important role in the development of vitreous hemorrhages.
出处
《眼科研究》
CSCD
北大核心
2003年第3期318-320,共3页
Chinese Ophthalmic Research
基金
河南省科技攻关项目基金资助 (0 0 1 1 70 637)
关键词
Eales’病
血管内皮生长因子
眼内液
酶联免疫吸附测定
Eales'disease vascular endothelial growth factor aqueous humor,vitreous/chemistry enzyme linked immunosorbent assay
