摘要
目的 定量研究肝细胞生长因子 (HGF)在增生性玻璃体视网膜病变 (PVR)患者玻璃体中的水平 .方法 采用双夹心酶联免疫吸附测定法检测正常对照组 10只眼、PVR- A,B组 7只眼、PVR- C组 13只眼、PVR- D组 16只眼 .结果 玻璃体内 HGF的含量 :正常对照组 1.8～ 7.4 μg· L- 1 ;PVR-A,B组 3.8～ 12 .8μg· L- 1 ;PVR- C组 7.7～ 2 0 .1μg· L- 1 ;PVR- D组 10 .3～ 2 4 .6μg· L- 1 .PVR各组玻璃体中 HGF的含量比正常对照组显著升高 (P<0 .0 1) ;PVR- C,D组玻璃体中 HGF的含量较 PVR- A,B组的含量升高 (P<0 .0 5 orP<0 .0 1) ;PVR- D组玻璃体中 HGF的含量与 PVR- C组比较含量也升高 (P<0 .0 5 ) ,玻璃体中 HGF的含量随着 PVR的发展而呈上行性升高 .结论 PVR患者玻璃体中 HGF含量升高 ,HGF可能参与了 PVR的病理过程 .
AIM To determine the mass concentration of hepatocyte growth factor (HGF) in the vitreous body of patients with proliferative vitreoretinopathy (PVR). METHODS The high sensitive sandwich enzyme linked immunosorbent assay (ELISA) was used to measure the level of HGF in vitreous bodies of 10 normal eyes, 7 PVR A, B eyes, PVR C 13 eyes, and 16 PVR D 16 eyes. RESULTS The mean value of HGF level in the vitreous body was 3.3 (range 1.8~7.4)μg·L -1 in the normal group; 7.4 (range 3.8 ~ 12.8) μg·L -1 in the PVR A, B group; 12.0(range 7.7 ~20.1) μg·L -1 in the PVR C group, 15.6 (range 10.3~ 24.6) μg·L -1 in PVR D group. HGF levels in the PVR groups were significantly higher than the level in the normal group ( P <0.01); the PVR C, D groups showed a higher mean vitreous HGF concentration than that in PVR A, B group ( P <0.05 or P <0.01); the level of HGF in the PVR D group was also higher than that in the PVR C group ( P <0.05). From grade A, B PVR to grade D PVR, a progressive increase was observed in the amount of HGF. CONCLUSION HGF levels in the vitreous body complicated with PVR were significantly higher than the level in normal group. HGF might be involved in the pathogenesis of PVR.
出处
《第四军医大学学报》
CAS
北大核心
2002年第14期1300-1302,共3页
Journal of the Fourth Military Medical University
关键词
增生性玻璃体视网膜病
玻璃体
肝细胞生长因子
酶联免疫吸附测定
vitreoretinopathy, proliferative
vitreous body
hepatocyte growth factor
enzyme linked immunosorbent assay
