摘要
目的 探讨镍对雌性大鼠生殖细胞的毒作用及其机制。方法 健康性成熟Wistar雌性大鼠腹腔注射硫酸镍 (NiSO4) 1 2 5 ,2 5 0 ,5 0 0mg/kg染毒 ,1次 /d ,连续 2 1d。染毒结束次日进行超数排卵和卵细胞体外培养 ,观察排卵数和存活情况 ,同时酶法测卵巢一氧化氮合酶 (NOS)活力和一氧化氮 (NO)含量。结果 染毒组动物超排卵总数和卵母细胞体外培养过程的各观察时相卵母细胞存活数均减少 ,卵巢NOS酶活力和NO量升高。结论 卵巢NOS活性增加、NO水平升高可能是致卵巢功能损害 ,卵细胞质量下降的机制之一。
Objective To study the toxic effect of nickel sulfate on the germ cells in female rats and its mechanism.Methods Nickel sulfate was injected intraperitoneally for Wistar female rats with sexual maturation daily at different doses (1.25,2.50,5.00 mg/kg)for 21 days .The number of superovulation and surviving ovum were observed through cell culture,Activity of nitric oxide synthase(NOS) and nitric oxide (NO) content in the ovary were measured.Results The number of superovulation and surviving ovum was lower than those in the control group;activity of NOS and NO content in the ovary of the rats given nickel sulfate were higher compared with control group.Conclusion The ovary of the female rats was damaged by peritoneal injection of nickel sulfate,the number of superovulation and surviving ovum decrease was possibly associated with the increase of activity of NOS and NO content in the ovary.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2004年第7期830-831,共2页
Chinese Journal of Public Health
