摘要
目的 观察酶解对猕猴红细胞形态及功能的影响 ,评价酶解后通用红细胞的安全性。方法 以基因重组的α 半乳糖苷酶体外酶解猕猴类人B型抗原 ,并以改良吸收放散试验鉴定酶解效果 ,检测酶解前后猕猴红细胞的形态和功能变化 ,流式细胞仪测定酶解红细胞在受体内的 2 4h存活率及半衰期 ,监测受体回输前后不同时期的血液、尿液生化指标。结果 经α 半乳糖苷酶酶解后的红细胞其B抗原被清除 ,形态及功能与酶解前无明显变化 ,在受体内的 2 4h存活率为 84 .6 %和 6 8.1 % ,半衰期为 7d和 8d ,血液、尿液等主要生化指标亦正常。结论 α 半乳糖苷酶酶解对猕猴红细胞的形态和功能无不良影响 。
Objective To observe the effects of enzymolysis of human B like antigen by α galactosidase treatment on the morphology and function of cynomolgus monkey red blood cell (RBC) and to evaluate the safety of the enzyme treated RBC in animal transfusion. Methods RBC with human B like antigen was treated by recombined α galactosidase and the effect was evaluated by absorption elution test. Meanwhile, morphology and function of the treated RBC were examined and compared with pre treatment RBC. α galactosidase treated RBC was labeled with FITC and then infused to blood group A cynomolgus monkeys. Flow cytometry was used to detect the survival of donor RBC in recipient’s blood. Blood chemistry and urinalysis of recipients were performed before and after transfusion. Results The human B like antigen of cynomolgus monkey RBC was obliterated by α galactosidase treatment and the treated RBC maintained normal morphology and function. Survival at 24 h after transfusion was 84.6% and 68.1%. T 1/2 were 7d and 8d versus 13d in the controls. There were no change in blood chemistry and urinalysis of the recipients. Conclusion Enzymolysis of cynomolgus monkey RBC does not affect the cellular function and morphology. Transfusion of the enzyme treated human B like RBC into human A like cynomolgus monkeys is safe.
出处
《中国输血杂志》
CAS
CSCD
2004年第4期231-234,共4页
Chinese Journal of Blood Transfusion
基金
军队"十五"科技攻关项目"血型改造制备通用型血的研究"( 2 0 0 0 2 5 2 910 )课题
