摘要
为探讨小鼠胚胎干细胞参与早期胚胎发育的潜能 ,以neo基因作为目的基因进行了胚胎干细胞的转染 ,经G41 8的筛选 ,获得表达neo基因的单克隆细胞 ,挑取部分克隆扩大 ,进行小鼠囊胚腔注射 ,再重新植入小鼠子宫 ,共注射了 60个囊胚 ,分别回输到 5只假孕小鼠 ,在子代中得到了携带有neo基因的嵌合体小鼠。通过对嵌合体小鼠各组织PCR分析发现 ,neo基因可以在皮肤、肝脏。
To explore the potentiality of the mouse ES cell line which is employed in our laboratory were transfected with neo gene and selected with G418. Some ES cell clones expressing neo gene were obtained. After ES clones were picked up and expanded, the ES cells were mircoinjected into blastocysts and implanted miceuterus. 60 blastocysts were implanted into 5 mice. Finally, 3 chimeric mice with neo gene were born. PCR was carried out to analyze the presentation of neo gene in tissues of chimeric mice. The results showed that many tissues harbored the neo gene, including skin, liver and blood et al.
基金
国家"863"计划 ( 1 0 2 -1 0 -0 4 -0 3)
国家自然科学基金重点项目 ( 39730 2 1 0 )
国家人类基因组南方中心(CNCS99M1 1 )
国家自然科学基金项目 ( 39670 30 2 )~~
